CELL-SHAPE AND MEMBRANE CHANGES IN 8-CELL MOUSE EMBRYO - PREREQUISITES FOR MORPHOGENESIS OF BLASTOCYST
CELL-SHAPE AND MEMBRANE CHANGES IN 8-CELL MOUSE EMBRYO - PREREQUISITES FOR MORPHOGENESIS OF BLASTOCYST
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DOI：
10.1016/0012-1606(75)90262-6
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发表时间：
1975-01-01
影响因子：
2.7
通讯作者：
ANDERSON, E
中科院分区：
文献类型：
--
作者：
DUCIBELLA, T;ANDERSON, E
在八细胞阶段，植入前小鼠胚胎的卵裂球经历了一个戏剧性的形状变化，压实，这被认为是必不可少的未来分离假定的细胞类型。本研究表明，致密化1）发生在体内，2）伴随着紧密连接和间隙连接的形成，3）在体外可被去钙培养基和细胞松弛素B（CCB）可逆地抑制。虽然微管经常被观察到在皮层区域的致密化进行，秋水仙胺和秋水仙素没有抑制作用。无钙培养基和CCB解离致密胚，超过50%的恢复在正常培养基中，分别在3和0.25小时。致密化的Ca 2+阈值约为0.1-0.2 mM，可能是正常细胞间粘附所需的。由于致密化标志着紧密连接形成的开始，并在桑椹胚阶段为闭锁小带的发育提供了必要的细胞与细胞并置，因此被认为是囊胚形态发生的初始步骤。此外，这项研究提供了手段，通过可逆抑制重编程压实，从而研究细胞决定的理论。
At the eight-cell stage, the blastomeres of the preimplantation mouse embryo undergo a dramatic shape change, compaction, which is considered essential to the future segregation of presumptive cell types. This investigation demonstrates that compaction 1) occursin vivo, 2) is accompanied by the formation of tight and gap junctions and 3) can be reversibly inhibitedin vitroby calcium-depleted medium and also by cytochalasin B (CCB). Although microtubules frequently are observed in cortical regions where compaction is proceeding, colcemid and colchicine have no inhibitory effect.Calcium-free medium and CCB dissociate compacted embryos, over 50% of which recover in normal medium in 3 and 0.25 hr, respectively. The Ca2+threshold for compaction is approximately 0.1–0.2 mMand may be required for normal intercellular adhesions. Since compaction marks the beginning of tight junction formation and provides the necessary cell-to-cell apposition for the development of the zonula occludens at the morula stage, it is considered to be the initial step in blastocyst morphogenesis. In addition, this investigation provides the means to reprogram compaction by reversible inhibition and thereby study theories of cell determination.