CHOLINERGIC PHARMACOLOGY OF HIPPOCAMPAL PYRAMIDAL CELLS - MICROIONTOPHORETIC STUDY
CHOLINERGIC PHARMACOLOGY OF HIPPOCAMPAL PYRAMIDAL CELLS - MICROIONTOPHORETIC STUDY
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DOI：
10.1016/0028-3908(76)90128-3
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发表时间：
1976-01-01
影响因子：
4.7
通讯作者：
AGHAJANIAN, GK
中科院分区：
文献类型：
--
作者：
BIRD, SJ;AGHAJANIAN, GK
研究了大鼠中枢神经系统胆碱能系统的药理特性。通过细胞外记录监测海马锥体细胞对不同烟碱和毒碱类药物微离子导入的反应。研究了毒扁豆碱类和烟碱类化合物之间的相互作用。乙酰胆碱和卡巴胆碱在烟碱和毒扁豆碱部位都有效，很容易产生海马锥体细胞的兴奋。毒扁豆碱激动剂毒扁豆碱、乙酰甲胆碱和苯二酚在低离子导入电流下刺激这些细胞。乙酰胆碱对锥体细胞的兴奋作用不受谷氨酸的影响，但可完全阻断乙酰胆碱对锥体细胞的兴奋。海马区锥体细胞对某些烟碱类化合物有反应。烟碱激动剂苯基三甲基铵可引起锥体细胞兴奋。假定的烟碱激动剂四甲基铵和烟碱拮抗剂二氢-β-乙酸乙二胺和加拉胺特异性地阻断了乙酰胆碱对这些细胞的兴奋。被研究的其他烟碱剂产生了乙酰胆碱兴奋的非特异性阻断，因为对谷氨酸的反应也受到影响。经典的烟碱拮抗剂(+)-Tubocurarine在所测试的量中没有产生任何作用。烟碱类拮抗剂二氢-β-乙二胺和加拉胺也完全阻断了毒碱激动剂毒鼠碱和乙酰甲基胆碱对锥体细胞的兴奋作用。由于烟碱拮抗剂和烟碱激动剂之间存在交叉，所以海马锥体细胞对毒碱和一些烟碱类药物有反应，这些药物不作用于两个独立的受体。
Pharmacological properties of an identified cholinergic system in the CNS of the rat were studied. The responses of hippocampal pyramidal cells to the microiontophoretic application of various nicotinic and muscarinic agents were monitored via extracellular recording. Interactions between muscarinic and nicotinic compounds were investigated. Acetylcholine and carbachol, agents effective at both nicotinic and muscarinic sites, readily produced excitation of hippocampal pyramidal cells. The muscarinic agonists muscarine, acetyl-.beta.-methylcholine and bethanechol excited these cells at low iontophoretic currents. Muscarinic antagonists scopolamine and quinuclidinyl benzilate totally blocked acetylcholine excitation of pyramidal cells without altering their excitation by glutamate. Hippocampal pyramidal cells were responsive to certain nicotinic compounds. The nicotinic agonist phenyltrimethylammonium caused excitation of pyramidal cells. The putative nicotinic agonist tetramethylammonium and the nicotinic antagonists dihydro-.beta.-erythroidine and gallamine specifically blocked acetylcholine excitation of these cells. Other nicotinic agents investigated produced a nonspecific block of acetylcholine excitation in that the response to glutamate was also affected. The classical nicotinic antagonist (+)-tubocurarine produced no effect in the amounts tested. The nicotinic antagonists dihydro-.beta.-erythroidine and gallamine also completely blocked the excitation of pyramidal cells by the muscarinic agonists muscarine and acetyl-.beta.-methylcholine. Hippocampal pyramidal cells are responsive to muscarinic and some nicotinic agents, and these agents are not acting at 2 independent receptors, since there is crossover between nicotinic antagonists and muscarinic agonists.