Variations in rRNA content of marine Vibrio spp. during starvation-survival and recovery
Variations in rRNA content of marine Vibrio spp. during starvation-survival and recovery
复制标题
海洋弧菌rRNA含量的变化。
DOI：
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发表时间：
1992
影响因子：
4.4
通讯作者：
F. Singleton
中科院分区：
文献类型：
--
作者：
Jonathan G. Kramer;F. Singleton
研究了两种共营养海洋细菌--溶藻弧菌和弗氏弧菌在营养饥饿过程中核糖体含量变化的程度和时间背景。这些生物体的饥饿要么是由于营养转移，要么是由于生长进入稳定期而消耗了有限的养分。对16S rRNA特异探针杂交量的测量表明，在饥饿15天后，细胞保留了原始rRNA含量的10%至26%。在溶藻弧菌中，静止期细胞的损失迅速发生(1-2天)，而进入饥饿状态的细胞仍然较大，并保留了相当多的rRNA。细胞在营养耗尽的培养液中维持2、8和15天后，对溶藻弧菌从饥饿中恢复的能力进行评估。RRNA积累水平的恢复模式取决于营养剥夺的持续时间和施加的方式。饥饿2天的静止期细胞在添加过量营养后，rRNA水平仅有轻微的相对增加。随着饥饿时间延长到8天和15天，营养强化后转录的rRNA越来越多(分别是预富集值的30倍和70倍)。下移细胞从饥饿的2天和8天中恢复过来，没有大量的rRNA产生。15天后，营养强化使16S rRNA水平增加了30倍。结果表明，这些海洋细菌物种控制饥饿-生存的机制与饥饿开始时的生理状态有关，随后的恢复模式将取决于饥饿是如何开始的。
The degree and temporal context of variations in ribosome content during nutrient starvation of two copiotrophic marine bacteria, Vibrio alginolyticus and Vibrio furnissii, have been examined. The organisms were starved either by nutritional shift-down or by consumption of limiting nutrients resulting from growth into stationary phase. Measurements of the amount of hybridization to 16S rRNA-specific probes revealed that the cells retained between 10 and 26% of their original rRNA content after 15 days of starvation. In V. alginolyticus, losses in stationary-phase cells occurred rapidly (1 to 2 days), whereas cells shifted into starvation remained larger and retained considerably more rRNA. The ability of V. alginolyticus to recover from starvation was assessed after cells were maintained for 2, 8, and 15 days in nutrient-depleted medium. The pattern of recovery at the level of rRNA accumulation depended upon the duration of nutrient deprivation and the manner in which it was imposed. Stationary-phase cells starved for 2 days had only slight relative increases in rRNA levels after excess nutrients were added. As the duration of starvation lengthened to 8 and 15 days, increasingly greater amounts of rRNA (30 and 70 times preenrichment values, respectively) were transcribed after nutrient enrichment. Shift-down cells recovered from 2 and 8 days of starvation without extensive rRNA production. After 15 days, nutrient enrichment caused 16S rRNA levels to increase 30-fold. The results indicate that the mechanisms controlling starvation-survival in these marine bacterial species are linked to the physiological state at the onset of starvation and that the subsequent pattern of recovery will depend upon how starvation was initiated.