TRANSFERRIN RECEPTORS IN RAT-BRAIN - NEUROPEPTIDE-LIKE PATTERN AND RELATIONSHIP TO IRON DISTRIBUTION
TRANSFERRIN RECEPTORS IN RAT-BRAIN - NEUROPEPTIDE-LIKE PATTERN AND RELATIONSHIP TO IRON DISTRIBUTION
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DOI：
10.1073/pnas.82.13.4553
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
PERT, CB
中科院分区：
文献类型：
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作者：
HILL, JM;RUFF, MR;PERT, CB
对 125I 标记的转铁蛋白与大鼠脑切片的结合进行了表征和可视化。这种可饱和、可逆、高亲和力（Kd=1×10-9M）结合位点似乎与先前在其他组织中表征的转铁蛋白受体没有区别。针对大鼠淋巴细胞转铁蛋白受体产生的单克隆抗体可以免疫沉淀从标记的脑切片中溶解的恢复的完整转铁蛋白，这表明标记是针对先前表征为转铁蛋白受体的相同分子实体。使用 125I 标记的转铁蛋白和抗转铁蛋白受体单克隆抗体在大脑中观察到的转铁蛋白受体分布模式几乎无法区分，但与 Fe 分布模式不同。富含铁的大脑区域通常接收来自转铁蛋白受体丰富的区域的神经元投射，这表明铁可能通过神经元转运。许多具有高密度转铁蛋白受体的大脑区域似乎与铁吸收和神经元转运无关，并形成与其他已知神经肽相似的受体分布模式。这种类似神经肽的分布模式表明，转铁蛋白可能在大脑中具有神经调节功能，或许还有行为功能。
The binding of 125I-labeled transferrin to sections of rat brain was characterized and visualized. This saturable, reversible, high-affinity (Kd = 1 .times. 10-9 M) binding site appears indistinguishable from transferrin receptors previously characterized in other tissues. A monoclonal antibody raised to rat lymphocyte transferrin receptors could immunoprecipitate recovered intact transferrin solubilized from labeled brain slices, indicating that labeling was to the same molecular entity previously characterized as the transferrin receptor. The pattern of transferrin receptor distribution visualized in brain with both 125I-labeled transferrin and an anti-transferrin receptor monoclonal antibody are almost indistinguishable but differ from the pattern of Fe distribution. Fe-rich brain areas generally receive neuronal projections from areas with abundant transferrin receptors, suggesting that Fe may be transported neuronally. Many brain areas with a high density of transferrin receptors appear unrelated to Fe uptake and neuronal transport and form a receptor distribution pattern similar to that of other known neuropeptides. This neuropeptide-like distribution pattern suggests that transferrin may have neuromodulatory, perhaps behavioral, function in brain.