Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a PHOSPHOLIPASE Dα1 knockout mutant
Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a PHOSPHOLIPASE Dα1 knockout mutant
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DOI：
10.1016/j.phytochem.2006.06.005
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发表时间：
2006-09-01
期刊：
影响因子：
3.8
通讯作者：
Wang, Xuemin
中科院分区：
文献类型：
--
作者：
Devaiah, Shivakumar Pattada;Roth, Mary R.;Wang, Xuemin
脂谱是一个具有针对性的代谢组学平台，它提供了对脂类的全面分析，具有很高的灵敏度。基于电喷雾电离串联质谱仪(ESI-MS/MS)的图谱提供了定量数据，适用于高通量分析。在这里，我们报告了拟南芥叶片、花柄、花、角果、根和种子中140个明显的极性甘油脂分子种类的概况。这些器官之间的脂质种类有相当大的差异，这为了解特定器官中不同的脂质代谢活动提供了洞察力。此外，野生型和敲除突变体PLDα1(位点ID：AT3G15730)之间的比较分析可以深入了解磷脂酶D(PLD)在不同器官中的代谢功能。PLDα1对根、种子、花和花梗中的磷脂酸(PA)水平有显著影响，但对角果和叶片中的基础PA水平影响不大。在PLDα1突变体的种子中，PA、溶血磷脂酰胆碱和溶血磷脂酰乙醇胺的水平显著低于野生型种子，表明PLDα1在种子膜脂降解中起作用。(C)2006爱思唯尔有限公司。保留所有权利。
Lipid profiling is a targeted metabolomics platform that provides a comprehensive analysis of lipid species with high sensitivity. Profiling based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) provides quantitative data and is adaptable to high throughput analyses. Here we report the profiling of 140 apparent molecular species of polar glycerolipids in Arabidopsis leaves, flower stalks, flowers, siliques, roots, and seeds. Considerable differences in lipid species occur among these organs, providing insights into the different lipid metabolic activities in a specific organ. In addition, comparative profiling between wild-type and a knockout mutant pld alpha 1 (locus ID: AT3G15730) provides insight into the metabolic function of phospholipase D (PLD) in different organs. PLD alpha 1 contributes significantly to phosphatidic acid (PA) levels in roots, seeds, flowers, and flower stalks, but little to basal PA levels in siliques and leaves. In seeds of the pld alpha 1 mutant plants, levels of PA, lysophosphatidylcholine, and lysophosphatidylethanolamine were significantly lower than those of wild-type seeds, suggesting a role for PLD alpha 1 in membrane lipid degradation in seeds. (c) 2006 Elsevier Ltd. All rights reserved.