Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity.
Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity.
复制标题
去势和睾酮给药对大鼠肝脏乙醇脱氢酶活性的影响。
DOI：
10.1016/0006-2952(80)90582-1
复制
发表时间：
1980
影响因子：
5.8
通讯作者：
Tsitouras,PD
中科院分区：
文献类型：
--
作者：
Mezey,E;Potter,JJ;Harmon,SM;Tsitouras,PD
本文测定了去势和睾酮给药对雄性SD大鼠肝脏乙醇脱氢酶活性和乙醇清除率的影响。去势后大鼠肝脏酒精脱氢酶活性升高。去势组大鼠肝脏总活度为2.37±0.229摩尔小时，而假手术对照组为1.39±0.125摩尔小时(P<0.01)。给予睾酮可部分抑制去势所产生的肝脏酒精脱氢酶活性的增强。相比之下，在对照组动物中，服用睾丸素导致肝脏酒精脱氢酶略有矛盾的增加。去势动物体内酶活性的增加与乙醇排泄率的增加是平行的。去势组和对照组在乙醇处理后胞浆游离NAD+/NADH和线粒体NAD+/NADH比值降低。这些观察表明，睾酮(可能还有其他未知的去势修饰因素)影响肝脏乙醇脱氢酶活性，总酶活性可能是乙醇消除的主要限制因素。
The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37±0.229 (SE) mmoles hr as compared with a value of 1.39±0.125 mmoles hr in sham-operated controls (P< 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.