Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells
Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells
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DOI：
10.1074/jbc.m707224200
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发表时间：
2008-01-11
影响因子：
4.8
通讯作者：
Lund, Anders H.
中科院分区：
文献类型：
--
作者：
Frankel, Lisa B.;Christoffersen, Nanna R.;Lund, Anders H.
microRNA正在成为癌症相关过程的重要调节因子。miR-21 microRNA在多种癌症中过表达，并与细胞增殖、凋亡和迁移有因果关系。在MCF-7乳腺癌细胞中抑制mir-21导致细胞生长减少。使用去除miR-21的MCF-7细胞的阵列表达分析，我们已经鉴定了miR-21的mRNA靶点，并显示了miR-21和p53肿瘤抑制蛋白之间的联系。我们还发现肿瘤抑制蛋白程序性细胞死亡4（PDCD 4）受miR-21调控，并证明PDCD 4是乳腺癌细胞中miR-21的重要功能靶点。
MicroRNAs are emerging as important regulators of cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety of cancers and has been causally linked to cellular proliferation, apoptosis, and migration. Inhibition of mir-21 in MCF-7 breast cancer cells causes reduced cell growth. Using array expression analysis of MCF-7 cells depleted of miR-21, we have identified mRNA targets of mir-21 and have shown a link between miR-21 and the p53 tumor suppressor protein. We furthermore found that the tumor suppressor protein Programmed Cell Death 4 (PDCD4) is regulated by miR-21 and demonstrated that PDCD4 is a functionally important target for miR-21 in breast cancer cells.