Effects of ethanolic extract from Radix Scrophulariae on ventricular remodeling in rats
Effects of ethanolic extract from Radix Scrophulariae on ventricular remodeling in rats
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DOI：
10.1016/j.phymed.2011.09.079
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发表时间：
2012-02-15
期刊：
影响因子：
7.9
通讯作者：
Li, Yi Ming
中科院分区：
文献类型：
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作者：
Huang, Xiao Yan;Chen, Chang Xun;Li, Yi Ming
目的：探讨玄参乙醇提取物（EERS）对大鼠心室重构的影响。方法：将冠脉结扎大鼠随机分为5组：冠脉结扎模型；CAL加卡托普利40 mg/kg；CAL加60毫克/公斤，120毫克/公斤，240毫克/公斤EERS。将假手术大鼠随机分为2组，分别为假手术对照组和假手术加EERS 120 mg/kg组。大鼠口服相应药物或饮水，连续14周。测定左室重量指数（LVWI）和心脏重量指数（HWI）。心肌组织分别用苏木精、伊红或苦味酸/天狼星红染色测定心肌细胞横截面积或胶原含量。测定心肌和血清中羟脯氨酸（Hyp）、基质金属蛋白酶2 （MMP-2）、血管紧张素II （Ang II）、醛固酮（ALD）、内皮素1 （ET-1）、心房利钠肽（ANP）、肿瘤坏死因子α (tnf - α)和肾素活性（RA）的浓度。采用Real-time RT-PCR检测血管紧张素转换酶（ACE）、ET-1、ANP mRNA的表达。结果：EERS能显著降低LVWI、HWI，降低心肌组织中Hyp浓度和胶原沉积，缩小心肌细胞横截面积，降低组织中Ang II、ET-1、ANP、tnf - α水平。EERS还能下调心肌组织中ACE、ET-1、ANP mRNA的表达。结论：EERS可减轻心室重构。其机制可能与抑制RAAS、tnf - α的过度激活以及调节与心脏肥厚相关的一些基因表达有关。(C) 2011爱思唯尔有限公司版权所有。
Purpose: To explore the effects of ethanolic extract of Radix Scrophulariae (EERS) on ventricular remodeling in rats.Methods: Rats with coronary artery ligation (CAL) were randomly assigned to 5 groups: CAL model; CAL plus 40 mg/kg captopril; CAL plus 60 mg/kg, 120 mg/kg, 240 mg/kg EERS. Sham operation rats were randomly assigned to 2 groups, sham-operated control and sham-operated plus 120 mg/kg EERS. The rats were orally administered with the corresponding drugs or drinking water for 14 weeks. The left ventricular weight index (LVWI) and heart weight index (HWI) were determined. Myocardium tissue was stained with hematoxylin and eosin or picric acid/Sirius red for cardiomyocyte cross-section area or collagen content measurements respectively. The concentrations of hydroxyproline (Hyp), matrix metalloproteinase 2 (MMP-2), angiotensin II (Ang II), aldosterone (ALD), endothelin 1 (ET-1), atrial natriuretic peptide (ANP), tumor necrosis factor alpha (TNF-alpha) and renin activity (RA) in myocardium or serum were determined. Real-time RT-PCR was used to detect the mRNA expressions of angiotensin converting enzyme (ACE), ET-1 and ANP.Results: EERS could significantly reduce the LVWI and HWI, decrease heart tissue concentrations of Hyp and collagen deposition, diminish cardiomyocyte cross-section area, reduce the tissue level of Ang II, ET-1, ANP and TNF-alpha. EERS could also down regulate the mRNA expression of ACE, ET-1 and ANP in myocardium.Conclusion: EERS attenuates ventricular remodeling. The mechanisms may be related to restraining the excessive activation of RAAS, TNF-alpha and modulating some gene expressions associated with cardiac hypertrophy. (C) 2011 Elsevier GmbH. All rights reserved.