PCR-based genotyping of the rat Atrn(mv) mutation.
PCR-based genotyping of the rat Atrn(mv) mutation.
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基于 PCR 的大鼠 Atrn(mv) 突变基因分型。
DOI：
10.1538/expanim.53.73
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发表时间：
2004
影响因子：
2.4
通讯作者：
T. Serikawa
中科院分区：
文献类型：
--
作者：
S. Tokuda;T. Kuramoto;T. Serikawa
大鼠吸引素基因座髓鞘空泡化突变（Atrn（mv））是一种包括Atrn基因的整个外显子1的基因组缺失。由于大鼠Atrn基因的基因组结构信息不足，缺失区域的精确大小和位置尚未确定。在这里，我们使用大鼠基因组的草图序列确定了Atrn（mv）突变的断点。在Atrn（mv/mv）大鼠中，6，914-bp的基因组区域被删除。引物侧翼的5 '-和3'-断点扩增Atrn（mv）等位基因，但不是野生型等位基因。该引物组使我们能够区分Atrn（mv/+）杂合子大鼠和Atrn（+/+）大鼠，并将有助于Atrn（mv/mv）大鼠的有效生产。
Rat myelin vacuolation mutation at the Attractin locus (Atrn(mv)) is a genomic deletion including the whole exon 1 of the Atrn gene. The precise size and location of the deleted region has not yet been identified because of poor information on genomic organization of the rat Atrn gene. Here, we identified the breakpoints of the Atrn(mv) mutation, using a draft sequence of the rat genome. In the Atrn(mv/mv) rat, a 6,914-bp genomic region was deleted. Primers flanked 5'- and 3'- breakpoints amplified the Atrn(mv) allele but not the wild-type allele. This primer set enables us to distinguish Atrn(mv/+) heterozygous rats from Atrn(+/+) rats, and will contribute to the efficient production of Atrn(mv/mv) rats.