Cloning and Characterization of a Critical Regulator for Preharvest Sprouting in Wheat
Cloning and Characterization of a Critical Regulator for Preharvest Sprouting in Wheat
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DOI：
10.1534/genetics.113.152330
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发表时间：
2013-09-01
期刊：
影响因子：
3.3
通讯作者：
Bai, Guihua
中科院分区：
文献类型：
--
作者：
Liu, Shubing;Sehgal, Sunish K.;Bai, Guihua
收获前成熟穗粒发芽是全世界小麦生产中的一个主要问题。利用比较作图和图位克隆技术，在白色小麦3A染色体短臂上克隆了一个抗穗发芽的小麦数量性状基因（QTL）。该基因被命名为TaPHS1，是小麦开花时间母亲（TaMFT）样基因的同源物。RNA干扰介导的基因敲低证实了TaPHS1正调控PHS抗性。我们在TaPHS1中发现了两个共同改变小麦PHS抗性的因果突变。一个GT到AT突变产生一个错误剪接位点，另一个A到T突变产生一个过早的终止密码子，导致截短的无功能转录本。对一组小麦品种的关联分析验证了这两个突变在PHS抗性中的作用。TaPHS1的分子鉴定对小麦生产中加快抗小穗病育种、保护籽粒产量和品质具有重要意义。
Sprouting of grains in mature spikes before harvest is a major problem in wheat (Triticum aestivum) production worldwide. We cloned and characterized a gene underlying a wheat quantitative trait locus (QTL) on the short arm of chromosome 3A for preharvest sprouting (PHS) resistance in white wheat using comparative mapping and map-based cloning. This gene, designated TaPHS1, is a wheat homolog of a MOTHER OF FLOWERING TIME (TaMFT)-like gene. RNA interference-mediated knockdown of the gene confirmed that TaPHS1 positively regulates PHS resistance. We discovered two causal mutations in TaPHS1 that jointly altered PHS resistance in wheat. One GT-to-AT mutation generates a mis-splicing site, and the other A-to-T mutation creates a premature stop codon that results in a truncated nonfunctional transcript. Association analysis of a set of wheat cultivars validated the role of the two mutations on PHS resistance. The molecular characterization of TaPHS1 is significant for expediting breeding for PHS resistance to protect grain yield and quality in wheat production.