Prevention effects of Schisandra polysaccharide on radiation-induced immune system dysfunction
Prevention effects of Schisandra polysaccharide on radiation-induced immune system dysfunction
复制标题
五味子多糖对辐射引起的免疫系统功能障碍的预防作用。
DOI：
10.1016/j.ijbiomac.2015.02.020
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发表时间：
2015-05-01
影响因子：
8.2
通讯作者：
Liu, Li-Hua
中科院分区：
文献类型：
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作者：
Zhao, Lian-Mei;Jia, Yun-Long;Liu, Li-Hua
本研究以Bal/bc小鼠为模型，探讨了五味子多糖（SP）对辐射诱导的免疫功能障碍的预防作用及其机制。结果表明，SP可逆转外周血中白色细胞和淋巴细胞数量的减少。此外，血清免疫球蛋白G（IgG）和补体C3在辐射后均下降，SP可恢复这种辐射紊乱。此外，SP还能逆转放疗后小鼠外周血和胸腺中CD 3（+）CD 4（+）和CD 3（+）CD 8（+）T细胞亚群的失调。我们还通过末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）和免疫组化（IHC）研究了胸腺中SP的凋亡及其机制。结果表明，SP可通过上调Bcl-2的表达，下调Fas和Box的表达，逆转辐射诱导的胸腺细胞凋亡。SP对正常小鼠的免疫功能无明显影响。提示SP可通过保护机体免疫系统，有效地预防放疗引起的免疫损伤。这些有价值的信息将有助于选择合理的治疗干预设计，以预防放射治疗中的免疫系统损伤。(C)2015年由Elsevier B. V.出版
In this study, we investigate the efficacy of SP (Schisandra polysaccharide) in prevention of radiation-induced immune dysfunction and discussed the underlying mechanisms with a Bal/bc mouse model. The data demonstrated that SP could reverse the decreases in the number of white blood cells and lymphocytes in peripheral blood. In addition, the immunoglobulin G (IgG) and complement C3 in blood serum were all decreased after radiation and SP could restore this radiation disorder. Furthermore, SP could reverse the deregulation of CD3(+)CD4(+) and CD3(+)CD8(+) T cell subsets in peripheral blood and thymus of mice after radiotherapy. We also performed terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) and Immunohistochemistry (IHC) to investigate the apoptosis and underlying mechanisms of SP in thymus. Data showed that radiation-induced apoptosis of thymocytes could be reversed by SP through inducing upregulation of Bcl-2 expression and downregulation of Fas and Box levels. Furthermore, SP has no any side-effects on immunity of normal mice. In conclusion, our results indicated that SP could effectively prevent immune injury during radiotherapy by protecting the immune system. This valuable information should be of assistance in choosing a rational design for therapeutic interventions of prevention immune system damage in the radiation treatment. (C) 2015 Published by Elsevier B.V.