A chicken transferrin binding protein is heat shock protein 108.
A chicken transferrin binding protein is heat shock protein 108.
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鸡转铁蛋白结合蛋白是热休克蛋白 108。
DOI：
10.1006/bbrc.1994.1414
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发表时间：
1994
影响因子：
3.1
通讯作者：
Lucas,JJ
中科院分区：
文献类型：
--
作者：
Hayes,GR;Himpler,BS;Weiner,KX;Lucas,JJ
通过对 RP-HPLC 纯化的胰蛋白酶肽进行微量测序，先前描述的来自鸡输卵管的转铁蛋白结合蛋白已被鉴定为热休克蛋白 HSP108。通过 SDS-PAGE 从输卵管纯化的蛋白质或通过卵转铁蛋白亲和层析从肝脏纯化的蛋白质与针对 HSP108 的单克隆抗体反应。 cDNA 序列预测羧基末端有 KDEL 肽，但该蛋白质不与抗 KDEL 单克隆抗体反应，表明 HSP108 的加工方式消除了 KDEL 表位。
A previously described transferrin binding protein from chicken oviduct has been identified as a heat shock protein, HSP108, by microsequencing of RP-HPLC purified tryptic peptides. The protein purified from oviduct by SDS-PAGE or from liver by ovotransferrin-affinity chromatography reacts with a monoclonal antibody raised against HSP108. The cDNA sequence predicts a KDEL peptide at the carboxyl terminus, but the protein does not react with anti-KDEL monoclonal antibodies, suggesting that HSP108 is processed in a way that eliminates the KDEL epitope.