IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF TESTICULAR AND SOMATIC CYTOCHROMES-C IN THE SEMINIFEROUS EPITHELIUM OF THE RAT
IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF TESTICULAR AND SOMATIC CYTOCHROMES-C IN THE SEMINIFEROUS EPITHELIUM OF THE RAT
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DOI：
10.1095/biolreprod48.6.1299
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发表时间：
1993-06-01
影响因子：
3.6
通讯作者：
GOLDBERG, E
中科院分区：
文献类型：
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作者：
HESS, RA;MILLER, LA;GOLDBERG, E
使用单特异性抗体通过免疫细胞化学将体细胞和睾丸特异性细胞色素c超微结构定位于生精上皮中。随着精子发生的进展，细胞色素 c(S) 从线粒体中消失，而在合子期到粗线期的转变过程中，细胞色素 c(T) 相对增加；这与其他研究一致，这些研究表明细胞色素 c(T) 基因在第一次减数分裂前期被激活。细胞色素 c(T) 高度集中在线粒体中，这些线粒体在精子细胞的细胞质叶内被降解，并且在被支持细胞吞噬的残留体中。在从细胞色素 c(S) 主导向细胞色素 c(T) 主导的过渡期间，发现这两种亚型共存于同一线粒体内。此外，两种细胞色素c都存在于精母细胞和圆形精子细胞的类染色体中；这表明类染色体可能参与这些同工酶的储存，并可能参与它们在生殖细胞线粒体内的差异表达。脱辅细胞色素c集中在生殖细胞的线粒体和类染色体中，也分散在细胞质中。精母细胞和精子细胞的类染色质体内存在全蛋白和脱辅基蛋白免疫探针，这是一个有趣的观察结果，它提出了关于生精细胞中细胞色素 c 合成的精确位置的问题。
Somatic and testis-specific cytochromes c were localized ultrastructurally in the seminiferous epithelium by immunocytochemistry using monospecific antibodies. Cytochrome c(S) was lost from the mitochondria as spermatogenesis advanced, while there was a relative increase in cytochrome c(T) during the zygotene-to-pachytene transition; this was in agreement with other studies that have suggested activation of the cytochrome c(T) gene during prophase of the first meiotic division. Cytochrome c(T) was highly concentrated in mitochondria that were being degraded within cytoplasmic lobes of spermatids and in residual bodies that were phagocytized by Sertoli cells. The two isoforms were found to coexist within the same mitochondrion during the transitional period from cytochrome c(S) to cytochrome c(T) predominance. In addition, both cytochromes c were present in the chromatoid bodies of spermatocytes and round spermatids; this suggests that the chromatoid body may be involved in the storage of these isozymes and possibly in their differential expression within germ cell mitochondria. Apocytochrome c was concentrated in mitochondria and chromatoid bodies of the germ cells and also scattered in the cytoplasm. The presence of the holoprotein and apoprotein immunoprobes within the chromatoid bodies of spermatocytes and spermatids was an interesting observation that raises questions regarding the precise location of the synthesis of cytochromes c in spermatogenic cells.