AN INTERCISTRONIC STEM-LOOP STRUCTURE FUNCTIONS AS AN MESSENGER-RNA DECAY TERMINATOR NECESSARY BUT INSUFFICIENT FOR PUF MESSENGER-RNA STABILITY
AN INTERCISTRONIC STEM-LOOP STRUCTURE FUNCTIONS AS AN MESSENGER-RNA DECAY TERMINATOR NECESSARY BUT INSUFFICIENT FOR PUF MESSENGER-RNA STABILITY
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DOI：
10.1016/0092-8674(88)90473-4
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发表时间：
1988-02-26
期刊：
影响因子：
64.5
通讯作者：
BELASCO, JG
中科院分区：
文献类型：
--
作者：
CHEN, CYA;BEATTY, JT;BELASCO, JG
puf操纵子的多顺反子转录本内的节段稳定性差异有助于R.囊状的这些转录本的相对稳定的5 '片段以大的顺反子间茎环结构结束。我们在这里表明，删除这个RNA发夹使5 'puf mRNA片段不稳定，但它在puf操纵子转录物的3'末端的插入不能稳定不稳定的3 'puf mRNA片段。有证据表明，衰变的3 '段开始与内切核酸裂解的顺反子间茎环结构不参与。我们的结论是，这种RNA发夹是必要的，但不足以稳定的mRNA上游，它的功能，在信息降解仅作为一个mRNA衰变终止子，保护上游mRNA片段降解的3 '外切核糖核酸酶。
Segmental differences in stability within the polycistronic transcripts of the puf operon contribute to differential expression of photosynthesis genes in R. capsulatus. The comparatively stable 5’segment of these transcripts ends in a large intercistronic stemloop structure. We show here that deletion of this RNA hairpin destabilizes the 5’puf mRNA segment but that its insertion at the 3’end of the puf operon transcripts fails to stabilize the labile 3’puf mRNA segment. Evidence is presented that decay of the 3’segment begins with endonucleolytic cleavage in which the Intercistronic stem-loop structure does not participate. We conclude that this RNA hairpin is necessary but insufficient for the stability of mRNA upstream of it, and that it functions in message degradation solely as an mRNA decay terminator that protects upstream mRNA segments from degradation by 3’exoribonucleases.