Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms
Caenorhabditis elegans mutants resistant to attachment of Yersinia biofilms
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DOI：
10.1534/genetics.106.067496
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发表时间：
2007-05-01
期刊：
影响因子：
3.3
通讯作者：
Drace, Kevin
中科院分区：
文献类型：
--
作者：
Darby, Creg;Chakraborti, Amrita;Drace, Kevin
秀丽隐杆线虫表面的详细组成和结构是未知的。先前的遗传学研究使用抗体或凝集素结合来鉴定在表面决定中起作用的srf基因。感染嗜热微杆菌确定总线（细菌不肿胀）基因，也影响表面特性。本文报道鼠疫菌和鼠疫耶尔森氏菌产生的生物膜。pseudotuberculosis，其结合C.线虫表面主要在头部，可用于识别额外的表面决定基因。一个屏幕为C。具有头部缺失生物膜（Bah）表型的秀丽线虫突变体鉴定了三种新基因：bah-1、bah-2和bah-3。的bah-1和bah-2突变体有轻微的脆弱的cured，但既不是Srf也不是BUS，这表明它们是特定的表面成分参与生物膜附着。bah-3突变体具有正常的角质层完整性，但显示出阶段特异性Srf表型。筛选产生了5个已知表面基因的等位基因：srf-2、srf-3、bus-4、bus-12和bus-17。对于X连锁的总线-17，在生物膜测定中观察到父系效应。
The detailed composition and structure of the Caenorhabditis elegans surface are unknown. Previous genetic studies used antibody or lectin binding to identify srf genes that play roles in surface determination. Infection by Microbacterium nematophilum identified bus (bacterially unswollen) genes that also affect Surface characteristics. We report that biofilms produced by Yersinia pestis and Y. pseudotuberculosis, which bind the C. elegans surface predominantly on the head, can be used to identify additional surface-determining genes. A screen for C. elegans mutants with a biofilm absent on the head (Bah) phenotype identified three novel genes: bah-1, bah-2, and bah-3. The bah-1 and bah-2 mutants have slightly fragile cuticles but are neither Srf nor BUS, suggesting that they are specific for surface components involved in biofilm attachment. A bah-3 mutant has normal cuticle integrity, but shows a stage-specific Srf phenotype. The screen produced alleles of five known surface genes: srf-2, srf-3, bus-4, bus-12, and bus-17. For the X-linked bus-17, a paternal effect was observed in biofilm assays.