Absorbance detection of amino acids by laser wave mixing in microbore liquid chromatography.
Absorbance detection of amino acids by laser wave mixing in microbore liquid chromatography.
复制标题
微孔液相色谱中激光波混合的氨基酸吸光度检测。
DOI：
10.1016/s0021-9673(98)00022-3
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Tong,WG
中科院分区：
文献类型：
--
作者：
Wu,Z;Tong,WG
利用简并四波混频的非线性光学相位共轭技术，可作为一种灵敏的微孔高效液相色谱“吸光度”检测方法。使用在488 nm线处操作的氩离子激光器作为激发光源以产生用于dabsyl标记的氨基酸的波混合信号。非线性激光检测方法的优点包括：几乎100%的光信号收集效率，相干激光束形式的信号的产生，相对于几乎暗的背景的信号测量，小吸光度值的可靠检测，对于发荧光和不发荧光的分析物的优异检测灵敏度，相对简单的单色单激光光学设置，以及对连续波或脉冲激光器的低功率或能量要求。使用我们的单激光单色非线性激光检测器的“吸光度”测量液相色谱法，我们报告了一个粗略的初步“注入”检测限为780 fmol甘氨酸。
Nonlinear optical phase conjugation by degenerate four-wave mixing is demonstrated as a sensitive “absorbance” detection method for microbore high-performance liquid chromatography. An argon ion laser operating at the 488-nm line is used as the excitation light source to generate the wave-mixing signal for dabsyl-labeled amino acids. Advantages of the nonlinear laser detection method include: virtually 100% optical signal collection efficiency, generation of the signal in the form of a coherent laser beam, signal measurement against a virtually dark background, reliable detection of small absorbance values, excellent detection sensitivity for both fluorescing and non-fluorescing analytes, relatively simple one-color one-laser optical setup, and low power or energy requirements for continuous-wave or pulsed lasers. Using our one-laser one-color nonlinear laser detector for “absorbance” measurements in liquid chromatography, we report a crude preliminary “injected” detection limit of 780 fmol for glycine.