Localization of the VIP2 Receptor Protein on GnRH Neurons in the Female Rat.
Localization of the VIP2 Receptor Protein on GnRH Neurons in the Female Rat.
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DOI：
10.1210/endo.141.11.7876
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发表时间：
2000-11
期刊：
影响因子：
4.8
通讯作者：
Matthew J. Smith;L. Jennes;P. Wise
中科院分区：
文献类型：
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作者：
Matthew J. Smith;L. Jennes;P. Wise
在哺乳动物中，排卵前LH峰的时间和发生关键取决于视交叉上核（SCN）的正常功能。最近的证据表明，血管活性肠多肽（VIP）传达的一天中的时间信息从SCN的GnRH神经元。然而，目前还不完全清楚这种作用是否直接在GnRH神经元水平发挥作用。为了确定GnRH神经元是否是VIP的直接靶点，利用三重标记的免疫荧光同时定位GnRH、VIP和VIP 2受体蛋白。目前的结果表明，约40%的所有GnRH神经元分析含有VIP 2受体免疫反应性和VIP的过程中被视为密切并列的VIP 2受体阳性GnRH神经元的显着数量。对VIP 2受体表现出免疫反应性的GnRH神经元主要位于OVLT区域和吻侧视前区。在正中隆起，大多数GnRH神经元终止，VIP 2接收。
In mammals, the timing and occurrence of the preovulatory LH surge critically depends on the proper functioning of the suprachiasmatic nucleus (SCN). Recent evidence suggests that vasoactive intestinal polypeptide (VIP) conveys time of day information from the SCN to GnRH neurons. However, it is not completely clear whether this action is exerted directly at the level of the GnRH neuron. To determine if GnRH neurons are direct targets for VIP, triple-lable immunofluorescence was utilized to simultaneously localize GnRH, VIP and VIP2 receptor protein. The present results demonstrate that about 40% of all GnRH neurons analyzed contain VIP2 receptor immunoreactivity and that VIP-containing processes were seen in close apposition to a significant number of VIP2 receptor-positive GnRH neurons. GnRH neurons that exhibit immunoreactivity for the VIP2 receptor are located predominantly in the OVLT region and the rostral preoptic area. In the median eminence, where the majority of GnRH neurons terminate, VIP2 rece...