N‐linked oligosaccharides are necessary and sufficient for association of glycosylated forms of bovine RNase with calnexin and calreticulin.
N‐linked oligosaccharides are necessary and sufficient for association of glycosylated forms of bovine RNase with calnexin and calreticulin.
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N-连接寡糖对于糖基化形式的牛 RNase 与钙联蛋白和钙网蛋白的结合是必要且充分的。
DOI：
10.1002/j.1460-2075.1996.tb01084.x
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
A. Helenius
中科院分区：
文献类型：
--
作者：
Aylin R. Rodan;Jan Fredrik Simons;Trombetta Es;A. Helenius
Calnexin and calreticulin are lectin‐like molecular chaperones that promote folding and assembly of newly synthesized glycoproteins in the endoplasmic reticulum. While it is well established that they interact with substrate monoglucosylated N‐linked oligosaccharides, it has been proposed that they also interact with polypeptide moieties. To test this notion, glycosylated forms of bovine pancreatic ribonuclease (RNase) were translated in the presence of microsomes and their folding and association with calnexin and calreticulin were monitored. When expressed with two N‐linked glycans in the presence of micromolar concentrations of deoxynojirimycin, this small soluble protein was found to bind firmly to both calnexin and calreticulin. The oligosaccharides were necessary for association, but it made no difference whether the RNase was folded or not. This indicated that unlike other chaperones, calnexin and calreticulin do not select their substrates on the basis of folding status. Moreover, enzymatic removal of the oligosaccharide chains using peptide N‐glycosidase F or removal of the glucoses by ER glucosidase II resulted in dissociation of the complexes. This indicated that the lectin‐like interaction, and not a protein‐protein interaction, played the central role in stabilizing RNase‐calnexin/calreticulin complexes.
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DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Margolese,L;Waneck,GL;Suzuki,CK;Degen,E;Flavell,RA;Williams,DB
通讯作者：
Williams,DB
影响因子：
11.4
作者：
Hebert, DN;Foellmer, B;Helenius, A
通讯作者：
Helenius, A
影响因子：
56.9
作者：
HAMMOND, C;HELENIUS, A
通讯作者：
HELENIUS, A
DOI：
--
发表时间：
1984-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Plummer;J. Elder;S. Alexander;A. W. Phelan;A. Tarentino
通讯作者：
T. Plummer;J. Elder;S. Alexander;A. W. Phelan;A. Tarentino
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DOI：
--
发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Carreno,BM;Schreiber,KL;McKean,DJ;Stroynowski,I;Hansen,TH
通讯作者：
Hansen,TH