Selective neuronal nitric oxide synthase inhibition blocks furosemide-stimulated renin secretion in vivo.
Selective neuronal nitric oxide synthase inhibition blocks furosemide-stimulated renin secretion in vivo.
复制标题
选择性神经元一氧化氮合酶抑制可阻断体内呋塞米刺激的肾素分泌。
DOI：
10.1152/ajprenal.1995.269.1.f134
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Beierwaltes,WH
中科院分区：
文献类型：
--
作者：
Beierwaltes,WH
致密黄斑是肾素的调节部位。它只含有一氧化氮合酶(NOS)的神经元亚型，这表明NO可以通过致密斑途径刺激肾素的分泌。为了测试神经元型一氧化氮合酶是否介导肾素的分泌，肾素被肾压力感受器或利尿剂速尿(通过致密斑途径作用)刺激。用7-硝基吲唑(7-NI，50 mg/kg ip)选择性阻断神经元型一氧化氮合酶(7-NI，50 mg/kg ip)前后，测定12只Inactin麻醉大鼠在正常(104+/-3 mm Hg)和降低肾灌流压力(65+/-1 mm Hg)下的肾素分泌率(RSR)。7-NI对基础血压(102+/-2 mm Hg)和肾血流量(RBF)无影响。降低肾灌流压力使RSR从11.8+/-3.3增加到22.9+/-5.7 ng Ang I.h-1.min-1(P<；0.01)(Ang I为血管紧张素I)。同样，在7-NI处理的大鼠中，减少灌注量使RSR从8.5+/-1.8增加到20.5+/-6.2 ng Ang I.H-1.min-1(P<；0.01)。测定12只对照大鼠和11只7-NI组大鼠静脉注射速尿5 mg/kg后的肾血流动力学和RSR。阻断神经元型一氧化氮合酶不会改变血压(102+/-2毫米汞柱)、RBF(5.8+/-0.4毫升·分-1克肾脏重量)或肾血管阻力(18.7+/-1.4毫升·毫升-1克肾脏重量)。
The macula densa is a regulatory site for renin. It contains exclusively the neuronal isoform of nitric oxide synthase (NOS), suggesting NO could stimulate renin secretion through the macula densa pathway. To test whether neuronal NOS mediates renin secretion, renin was stimulated by either the renal baroreceptor or the diuretic furosemide (acting through the macula densa pathway). Renin secretion rate (RSR) was measured in 12 Inactin-anesthetized rats at normal (104 +/- 3 mmHg) and reduced renal perfusion pressure (65 +/- 1 mmHg), before and after selective blockade of the neuronal NOS with 7-nitroindazole (7-NI, 50 mg/kg ip). 7-NI had no effect on basal blood pressure (102 +/- 2 mmHg) or renal blood flow (RBF). Decreasing renal perfusion pressure doubled RSR from 11.8 +/- 3.3 to 22.9 +/- 5.7 ng ANG I.h-1.min-1 (P < 0.01) (ANG I is angiotensin I). Similarly, in 7-NI-treated rats, reduced perfusion doubled RSR from 8.5 +/- 1.8 to 20.5 +/- 6.2 ng ANG I.h-1.min-1 (P < 0.01). Renal hemodynamics and RSR were measured in response to 5 mg/kg iv furosemide in 12 control rats and 11 rats treated with 7-NI. Blocking neuronal NOS did not alter blood pressure (102 +/- 2 mmHg), RBF (5.8 +/- 0.4 ml.min-1.g kidney wt-1), or renal vascular resistance (18.7 +/- 1.4 mmHg.ml-1.min.g kidney wt).(ABSTRACT TRUNCATED AT 250 WORDS)