Prevalence and molecular characterization of bovine coenurosis from Eastern Anatolian region of Turkey
Prevalence and molecular characterization of bovine coenurosis from Eastern Anatolian region of Turkey
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DOI：
10.1016/j.vetpar.2010.10.033
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发表时间：
2011-02-28
影响因子：
2.6
通讯作者：
Balkaya, I.
中科院分区：
文献类型：
--
作者：
Avcioglu, H.;Yildirim, A.;Balkaya, I.
本研究旨在了解2009年11月至2010年4月土耳其东部Erzurum省牛多头带绦虫的分生线虫--脑球绦虫的流行情况和分子特征。在1045头牛脑中发现5头牛(0.47%)感染牛脑包囊。所有包囊均可见脑圆线虫的特征和形态。对来自3头感染牛的细胞色素T细胞进行了遗传分析，经NaD_1和COX_1线粒体基因序列分析，证实为多头拟毛囊线虫。Erzurum分离株与GenBank中其他分离株的NaD1序列的差异在0.6-2.9%之间，而COX1序列的差异在0.2-2.6%之间。根据这两个基因，从系统发育分析可以看出，来自Erzurum省的3个分离物都属于同一群。目前的发现可能会为利用线粒体序列数据集对该地区鲜为人知的带绦虫的系统关系和流行病学的未来研究提供刺激。(C)2010爱思唯尔B.V.保留所有权利。
This study was conducted to determine the prevalence and molecular characteristics of Coenurus cerebralis, the metacestode of Taenia multiceps in cattle from Erzurum province located in eastern region of Turkey between November 2009 and April 2010. Five of 1045 brains of cattle (0.47%) were found to be infected with Coenurus cysts. The characteristics and morphology of C. cerebralis were seen in all the cysts. The cyts from three infected cattle were genetically analyzed and confirmed to be T. multiceps metacestodes by NAD1 and COX1 mitochondrial gene sequence analysis. Pairwise comparison between the NAD1 sequences of the T. multiceps isolates from Erzurum and other T. multiceps isolates available in GenBank showed differences ranging from 0.6 to 2.9%, while COX1 sequences showed differences ranging from 0.2 to 2.6%. Considering the two genes, it was seen that all of the three isolates from Erzurum province were in the same group according to phylogenetic analyses. The present findings could provide a stimulus for future studies on the systematic relationships and epidemiology of lesser-known taeniid cestodes in the region, employing mitochondrial sequence data sets. (C) 2010 Elsevier B.V. All rights reserved.