Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Rabbits in Xinjiang, China
Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Rabbits in Xinjiang, China
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中国新疆兔体内隐孢子虫、十二指肠贾第鞭毛虫和比氏肠细胞虫的分子特征
DOI：
10.1111/jeu.12629
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发表时间：
2018-11-01
影响因子：
2.2
通讯作者：
Zhang, Longxian
中科院分区：
文献类型：
--
作者：
Zhang, Xiangqian;Qi, Meng;Zhang, Longxian
从中国新疆维吾尔自治区的 10 个养殖场采集了 321 份兔子粪便样本。样本中隐孢子虫、十二指肠贾第鞭毛虫和比氏肠细胞虫的患病率分别为 3.4% (11/321)、1.9% (6/321) 和 2.8% (9/321)。小亚基核糖体 RNA (SSU rRNA) 基因序列分析将所有 11 个隐孢子虫阳性样本鉴定为 C. cuniculus。基于 60-kDa 糖蛋白基因座 (gp60) 的进一步亚型鉴定出 5 个 C. cuniculus 分离株为 VbA24 亚型。通过SSU rRNA、磷酸三糖异构酶、β-giardin和谷氨酸脱氢酶位点的多位点序列分型确定十二指肠球菌基因型，证实6个十二指肠球菌分离物属于组合B的BIV亚型。内转录间隔区分析表明，5个、3个和1个E. bieneusi分离物属于基因型J、BEB8、和 IV 型。这些结果表明，从中国兔子身上分离出来的隐孢子虫、十二指肠隐孢子虫和比氏埃里虫具有人畜共患的潜力。
A total of 321 rabbit fecal samples were collected from 10 farms in the Xinjiang Uyghur Autonomous Region, China. The prevalence of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in the samples was 3.4% (11/321), 1.9% (6/321), and 2.8% (9/321), respectively. Small subunit ribosomal RNA (SSU rRNA) gene sequence analysis identified all 11 Cryptosporidium‐positive samples as C. cuniculus. Further subtyping based on the 60‐kDaglycoprotein locus (gp60) identified five of the C. cuniculus isolates as subtype VbA24. G. duodenalis genotypes were determined by multilocus sequence typing of the SSU rRNA, triosephosphate isomerase, β‐giardin and glutamate dehydrogenase loci, which confirmed that six G. duodenalis isolates belonged to subtype BIV of assemblage B. Analysis of the internal transcribed spacer region, showed that five, three, and one E. bieneusi isolates belonged to genotypes J, BEB8, and Type IV, respectively. These results suggest that Cryptosporidium spp., G. duodenalis, and E. bieneusi isolates from rabbits in China have zoonotic potential.