NITRIC OXIDE ASSOCIATED PROTEIN1 (AtNOA1) is necessary for copper-induced lateral root elongation in Arabidopsis thaliana
NITRIC OXIDE ASSOCIATED PROTEIN1 (AtNOA1) is necessary for copper-induced lateral root elongation in Arabidopsis thaliana
复制标题
一氧化氮相关蛋白 1 (AtNOA1) 对于铜诱导拟南芥侧根伸长是必需的
DOI：
10.1016/j.envexpbot.2021.104544
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发表时间：
2021
影响因子：
5.7
通讯作者：
Xiao Zhang
中科院分区：
文献类型：
--
作者：
Qing-ping Zhao;Jing Wang;Hong-ru Yan;Meng-ya Yang;Jin Wang;Xiang Zhao;Xiao Zhang
铜（Cu 2+）是植物正常生长发育所必需的微量元素，但过量时具有很强的毒性。抑制主根伸长和刺激侧根（LR）的形成是通常报道的症状时，暴露于高浓度的Cu 2+。Cu ~（2+）诱导LR形成的机制还有待进一步阐明。在这里，我们报告说，铜+诱导的LR的形成是由一氧化氮相关蛋白1（AtNOA 1），主要是通过介导铜+诱导的LR伸长生长，但不是LR原基的启动。AtNOA 1突变抑制LR原基的启动。转录水平分析显示，Atnoa 1与WT之间在Cu ~（2+）稳态和耐受相关基因的表达上存在显著差异。生长素反应报告植物（DR 5：GUS和DR 5：GFP）表明，Cu ~（2+）诱导的生长素再分配在Atnoa 1中受到抑制。此外，Cu ~（2+）诱导的LR形成对外源生长素转运抑制剂NOA和NPA在Atnoa 1中比在WT中更敏感。有趣的是，Cu 2+诱导的H2 O2水平在Atnoa 1中高于WT，但Cu 2+诱导的LR形成在H2 O2相关突变体中是正常的。这些结果表明AtNOA 1调控拟南芥LR原基的起始和介导Cu ~（2+）诱导的LR伸长。AtNOA 1可能通过影响Cu ~（2+）诱导的生长素再分配和Cu ~（2+）诱导的植物体内稳态来介导Cu ~（2+）诱导的LR伸长，但不依赖于Cu ~（2+）诱导的H_2O_2积累。
Copper (Cu2+) is an essential metal micronutrients required for normal plant growth and development, but is highly toxic when in excess. Inhibition of primary root elongation and stimulation of lateral root (LR) formation are usual reported symptoms when exposure to high concentrations of Cu2+. The mechanism underlying Cu2+-induced LR formation remains to be further elucidated. Here, we reported that Cu2+-induced LR formation is regulated by NITRIC OXIDE ASSOCIATED PROTEIN1 (AtNOA1), mainly through mediating Cu2+-induced LR elongation growth, but not LR primordium initiation. The initiation of LR primordium is inhibited by the mutation ofAtNOA1. Transcriptional analysis displayed that the expression of genes involved in Cu2+homeostasis and tolerance showed significant difference betweenAtnoa1and WT. Cu2+-induced auxin redistribution was inhibited inAtnoa1, indicated by auxin response reporter plants (DR5:GUS and DR5:GFP). Furthermore, Cu2+-induced LR formation were more sensitive to exogenous auxin transport inhibitors NOA and NPA inAtnoa1than in WT. Interestingly, Cu2+-induced H2O2level was higher inAtnoa1than WT, but Cu2+-induced LR formation was normal in H2O2-related mutants. These results showed that AtNOA1 regulated the initiation of LR primordium and mediates Cu2+-induced LR elongation inArabidopsis thaliana. AtNOA1 mediates Cu2+-induced LR elongation maybe through affecting Cu2+homeostasis and Cu2+-induced auxin redistribution, but not depending on Cu2+-induced H2O2accumulation.