The Cyclic Di-GMP Phosphodiesterase Gene Rv1357c/BCG1419c Affects BCG Pellicle Production and In Vivo Maintenance
The Cyclic Di-GMP Phosphodiesterase Gene Rv1357c/BCG1419c Affects BCG Pellicle Production and In Vivo Maintenance
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DOI：
10.1002/iub.1353
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发表时间：
2015-02-01
期刊：
影响因子：
4.6
通讯作者：
Waters, Christopher M.
中科院分区：
文献类型：
--
作者：
Alberto Flores-Valdez, Mario;de Jesus Aceves-Sanchez, Michel;Waters, Christopher M.
生活在表面附着群落中的细菌，其中包含异质群体，涂有细胞外基质，并表现出耐药性（生物膜），通常与慢性感染有关。在分枝杆菌中，菌膜生长模式等同于体外生物膜并满足上述几个标准，而结核感染则呈现慢性（潜伏）感染阶段。由于分枝杆菌缺乏控制其他微生物产生生物膜所需的大多数基因，因此我们在牛分枝杆菌 BCG 中删除或表达了 hsp60 强启动子，这是唯一已知的 c-di-GMP 磷酸二酯酶 (PDE) 基因。 We found changes in pellicle production, cellular protein profiles, lipid production, resistance to nitrosative stress and maintenance in lungs and spleens of immunocompetent BALB/mice.我们的结果表明，BCG 中的表膜生成和保留在宿主体内的能力是相关的。 (c) 2015 IUBMB 生活，67(2):129-138，2015
Bacteria living in a surface-attached community that contains a heterogeneous population, coated with an extracellular matrix, and showing drug tolerance (biofilms) are often linked to chronic infections. In mycobacteria, the pellicle mode of growth has been equated to an in vitro biofilm and meets several of the criteria mentioned above, while tuberculosis infection presents a chronic (latent) phase of infection. As mycobacteria lack most genes required to control biofilm production by other microorganisms, we deleted or expressed from the hsp60 strong promoter the only known c-di-GMP phosphodiesterase (PDE) gene in Mycobacterium bovis BCG. We found changes in pellicle production, cellular protein profiles, lipid production, resistance to nitrosative stress and maintenance in lungs and spleens of immunocompetent BALB/mice. Our results show that pellicle production and capacity to remain within the host are linked in BCG. (c) 2015 IUBMB Life, 67(2):129-138, 2015