Rescue effects in radiobiology: Unirradiated bystander cells assist irradiated cells through intercellular signal feedback
Rescue effects in radiobiology: Unirradiated bystander cells assist irradiated cells through intercellular signal feedback
复制标题
放射生物学中的救援作用：未受辐射的旁观者细胞通过细胞间信号反馈协助受辐射的细胞
DOI：
10.1016/j.mrfmmm.2010.10.011
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发表时间：
2011-01-10
影响因子：
2.3
通讯作者：
Yu, K. N.
中科院分区：
文献类型：
--
作者：
Chen, S.;Zhao, Y.;Yu, K. N.
Mammalian cells respond to ionization radiation by sending out extracellular signals to affect non-irradiated neighboring cells, which is referred to as radiation induced bystander effect. In the present paper, we described a phenomenon entitled the "rescue effects", where the bystander cells rescued the irradiated cells through intercellular signal feedback. The effect was observed in both human primary fibroblast (NHLF) and cancer cells (HeLa) using two-cell co-culture systems. After co-culturing irradiated cells with unirradiated bystander cells for 24 h, the numbers of 53BP1 foci, corresponding to the number of DNA double-strand breaks in the irradiated cells were less than those in the irradiated cells that were not co-cultured with the bystander cells (0.78 +/- 0.04 foci/cell vs. 0.90 +/- 0.04 foci/cell) at a statistically significant level. Similarly, both micronucleus formation and extent of apoptosis in the irradiated cells were different at statistically significant levels if they were co-cultured with the bystander cells. Furthermore, it was found that unirradiated normal cells would also reduce the micronucleus formation in irradiated cancer cells. These results suggested that the rescue effects could participate in repairing the radiation-induced DNA damages through a media-mediated signaling feedback, thereby mitigating the cytotoxicity and genotoxicity of ionizing radiation. (C) 2010 Elsevier B.V. All rights reserved.
