Immunomodulation of ABA function affects early events in somatic embryo development
Immunomodulation of ABA function affects early events in somatic embryo development
复制标题
ABA 功能的免疫调节影响体细胞胚胎发育的早期事件
作者：
S. Senger;Hans;Udo Conrad;R. Manteuffel
摘要本文首次用脱落酸（阿坝）对烟草体细胞胚胎发生的免疫调节作用，研究了ABA对烟草早期胚胎发育的影响。 建立了在内质网中组成型表达抗脱落酸（阿坝）单链片段可变抗体的纯合转基因系。比较了转基因株系和野生型的体细胞胚发育情况。阿坝生物合成突变体aba1和aba2以及用阿坝生物合成抑制剂氟啶酮处理的野生型培养物也用于比较研究。胚胎结构的发育在阿坝功能被阻断或ABA缺乏的所有培养物的早期阶段都受到干扰。在阿坝补充的体外细胞培养物的正常体细胞胚发育恢复。
Abstract Immunomodulation of abscisic acid (ABA) function during somatic embryogenesis of Nicotiana plumbaginifolia has been used to demonstrate for the first time the effect of this phytohormone on early embryonic events. A homozygous transgenic line constitutively expressing an anti-abscisic acid (ABA) single chain fragment variable antibody in the endoplasmic reticulum was established. Development of somatic embryos from the transgenic line and the wild type was compared. The ABA biosynthesis mutants aba1 and aba2 and wild type cultures treated with the ABA biosynthesis inhibitor fluridone were also used for the comparative investigations. The development of embryonic structures was disturbed in the early stages of all cultures in which ABA function was blocked or which were ABA-deficient. After ABA complementation of the in vitro cell cultures normal somatic embryo development was restored.