Phytolith morphology research on wild and domesticated rice species in East Asia
Phytolith morphology research on wild and domesticated rice species in East Asia
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DOI：
10.1016/j.quaint.2012.02.013
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发表时间：
2013-02-21
影响因子：
2.2
通讯作者：
Pearsall, Deborah M.
中科院分区：
文献类型：
--
作者：
Gu, Yansheng;Zhao, Zhijun;Pearsall, Deborah M.
东亚地区水稻种中植硅体的系统描述很少。为了探讨植硅体形态多样性与分类学和基因组之间的关系，本研究在统计和聚类分析的基础上，对野生稻和驯化稻的植硅体形态进行了比较研究。所有的形态参数在500倍的放大倍数下用蔡司光学显微镜测量。用SPSS10.0软件进行三维作图和聚类分析。栽培稻和野生稻的叶片和花序含有丰富多样的植硅体类型，包括长细胞、短细胞、泡状细胞、毛细胞、不规则表皮细胞、叶肉和维管组织。对植硅体形态的比较研究表明，在物种水平上，长细胞、短细胞、泡状细胞、毛细胞和维管组织之间的重叠最多。隆突、十字、长细胞、毛细胞、副足泡状细胞、管胞和维管组织无分类价值。复杂的鞍座和不规则的表皮植硅体可能是对以前没有描述过的水稻物种的诊断。通过对野生稻和野生稻三种植硅体形态特征的比较研究，证实了双峰型颖片能成功地将野生稻和野生稻区分开来。对双叶藻、楔形泡状细胞和双峰型颖片细胞的所有形态参数进行的系统聚类分析表明，植硅体组合似乎受遗传控制，因此反映了分类学意义。研究结果对植物生理学、水稻栽培和环境考古具有重要意义。(C)2012年爱思唯尔有限公司和INQUA。版权所有。
Systematical descriptions of phytoliths from rice species in East Asia are scarce. In order to investigate the relationship among diversity of phytolith morphology, taxonomy and genome, comparative research on phytolith morphology of the wild and domesticated rice species is first performed on the basis of statistics and cluster analysis. All morphological parameters were measured at 500x magnification using a Zeiss light microscope. 3-D plots and cluster analysis are performed by SPSS 10.0 software. The leaves and inflorescences of domesticated and wild rice contain a great diversity of phytolith types including long cells, short cells, bulliform cells, hair cells, irregular epidermal cells, mesophyll and vascular tissues. Comparative research on phytolith morphology demonstrates that most overlap occurs among long cells, short cells, bulliform cells, hair cells and vascular tissues at the species level. Rondels, crosses, long cells, hair cells, parallepipedal bulliform cells, tracheid and vascular tissue exhibit no taxonomical value. Complex saddles and irregular epidermal phytoliths might be diagnostic to the rice species that had not been described before. Further comparative research on the morphological features of three phytolith types from the wild and domesticated rice species has confirmed that double-peaked glume cells measurements can separate domesticated Oryza species from wild ones successfully. Hierarchical cluster analysis on all morphological parameters of bilobates, cuneiform bulliform cells and double-peaked glume cells strongly demonstrates that phytolith assemblage appears to be under genetic control and therefore reflect taxonomical significance. The results are significant for plant physiology, rice cultivation and environmental archaeology. (C) 2012 Elsevier Ltd and INQUA. All rights reserved.