In vivo MRI for effective non-invasive detection and follow-up of an orthotopic mouse model of lung cancer
In vivo MRI for effective non-invasive detection and follow-up of an orthotopic mouse model of lung cancer
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DOI：
10.1002/nbm.3142
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发表时间：
2014-08-01
影响因子：
2.9
通讯作者：
Cremillieux, Yannick
中科院分区：
文献类型：
--
作者：
Bianchi, Andrea;Dufort, Sandrine;Cremillieux, Yannick
肺癌预后不佳的主要原因之一与肺癌的晚期诊断有关。在这项研究中，我们评估了潜在的优化的肺MRI技术作为一个完全非侵入性的方法，非小细胞肺癌（NSCLC）的MRI在体内检测和后续的小鼠模型的肺腺癌表达荧光素酶基因。生物发光的肺肿瘤细胞原位植入免疫缺陷小鼠。使用呼吸门控和自由呼吸协议将超短回波时间（UTE）MRI自由呼吸采集与标准梯度回波肺部MRI（FLASH）进行比较。MRI结果与生物发光成像（BLI）和金标准组织病理学分析进行了验证。在所有小鼠中，门控FLASH和非门控UTE MRI均成功识别出腺癌样病理组织，并且在MRI、BLI和组织学分析之间发现了良好的肿瘤共定位。在测量的生物发光信号和分别用UTE MRI或门控FLASH MRI量化的总肿瘤体积之间发现了极好或良好的相关性。当肿瘤在非门控MR FLASH图像上被分割时，没有发现显著的相关性。MRI被证明是一种强大的成像工具，能够检测、量化和纵向监测亚毫米非小细胞肺癌的发展。据我们所知，这是第一个研究，证明了一个完全无创的MRI定量检测肺腺癌在自由呼吸的小鼠的可行性。不存在电离辐射和MRI的高分辨率，以及拟议的非门控协议的完全非侵入性和良好的再现性，使该成像工具成为直接平移应用的理想选择。沿着。版权所有c（C）2014约翰威利父子有限公司
One of the main reasons for the dismal prognosis of lung cancer is related to the late diagnosis of this pathology. In this study, we evaluated the potential of optimized lung MRI techniques as a completely non-invasive approach for non-small-cell lung cancer (NSCLC) MRI in vivo detection and follow-up in a mouse model of lung adenocarcinoma expressing the luciferase gene.Bioluminescent lung tumour cells were orthotopically implanted in immuno-deficient mice. Ultra-short echo-time (UTE) MRI free-breathing acquisitions were compared with standard gradient-echo lung MRI (FLASH) using both respiratory-gated and free-breathing protocols. The MRI findings were validated against bioluminescence imaging (BLI) and gold-standard histopathology analysis.Adenocarcinoma-like pathological tissue was successfully identified in all the mice with gated-FLASH and non-gated UTE MRI, and good tumour co-localization was found between MRI, BLI and histological analyses. An excellent or good correlation was found between the measured bioluminescent signal and the total tumour volumes quantified with UTE MRI or gated-FLASH MRI, respectively. No significant correlation was found when the tumours were segmented on non-gated MR FLASH images.MRI was shown to be a powerful imaging tool able to detect, quantify and longitudinally monitor the development of sub-millimetric NSCLCs. To our knowledge, this is the first study which proves the feasibility of a completely non-invasive MRI quantitative detection of lung adenocarcinoma in freely breathing mice. The absence of ionizing radiation and the high-resolution of MRI, along with the complete non-invasiveness and good reproducibility of the proposed non-gated protocol, make this imaging tool ideal for direct translational applications. Copyright c (C) 2014 John Wiley & Sons, Ltd.