Epitope mapping by surface plasmon resonance in the BIAcore
Epitope mapping by surface plasmon resonance in the BIAcore
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DOI：
10.1007/bf02752698
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发表时间：
1998-02-01
影响因子：
2.6
通讯作者：
Johne, B
中科院分区：
文献类型：
--
作者：
Johne, B
表位可以定义为抗原模块上的特异性位点，其特征在于结合一种单克隆抗体（MAb）。可以在BIAcore生物传感器中通过表面等离子体共振进行表位作图以表征抗原或一组特异性MAb或两者。本文介绍了BIAcore仪器和方法，这样的映射。例子包括与简单和复杂的蛋白质，如肌红蛋白和钙卫蛋白，分别分子相互作用的研究。
An epitope may be defined as a specific site on an antigen module characterized by the binding of one monoclonal antibody (MAb). Epitope mapping by surface plasmon resonance in the BIAcore biosensor may be performed to characterize an antigen or a group of specific MAbs or both. This article describes the BIAcore instrument and methods for such mapping. Examples include molecular interaction studies with simple and complex proteins, such as myoglobin and calprotectin, respectively.