Hepatitis B virus X protein enhances NFκB activity through cooperating with VBP1
Hepatitis B virus X protein enhances NFκB activity through cooperating with VBP1
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DOI：
10.5483/bmbrep.2008.41.2.158
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发表时间：
2008-02-29
期刊：
影响因子：
3.8
通讯作者：
Ahn, Jeong Keun
中科院分区：
文献类型：
--
作者：
Kim, Sang Yong;Kim, Jin Chul;Ahn, Jeong Keun
B型肝炎病毒X蛋白（HBx）是B型肝炎病毒感染所必需的，对多种细胞机制发挥多效性作用。HBx也被证明是各种不同病毒和细胞启动子的间接转录反式激活因子。此外，HBx参与各种肝脏疾病的发展，包括肝细胞癌。然而，HBx在肝细胞癌发生中的机制仍不清楚。在本研究中，我们进行了酵母双杂交试验，以鉴定可能与HBx相互作用的新的细胞蛋白。我们获得了几个可能的细胞伙伴，包括VBP1，VHL肿瘤抑制蛋白的结合因子。免疫沉淀法证实HBx与VBP1在体内外均存在直接的物理相互作用。此外，我们发现VBP1促进HBx诱导的NF κ B B活化和细胞增殖。这些结果暗示HBx通过其与VBP1的相互作用在肝细胞癌的发展中发挥重要作用。
Hepatitis B virus X protein (HBx) is essential for hepatitis B virus infection and exerts a pleiotropic effect on various cellular machineries. HBx has been also demonstrated as an indirect transcriptional transactivator of various different viral and cellular promoters. In addition, HBx is involved in the development of various liver diseases including hepatocellular carcinoma. However the mechanism of HBx in hepatocellular carcinogenesis remains largely unknown. in this study, to identify possible new cellular proteins interacting with HBx, we carried out yeast two-hybrid assay. We obtained several possible cellular partners including VBP1, a binding factor for VHL tumor suppressor protein. The direct physical interaction between HBx and VBP1 in vitro and in vivo was confirnmed by immunoprecipitation assay. In addition, we found that VBP1 facilitates HBx-induced NF kappa B activation and cell proliferation. These results implicate the important role of HBx in the development of hepatocellular carcinoma through its interaction with VBP1.