Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4 Å resolution
Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4 Å resolution
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2.4 Å 分辨率的人类线粒体单链 DNA 结合蛋白的晶体结构
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
C. Kang
中科院分区：
文献类型：
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作者：
Cheng Yang;U. Curth;C. Urbanke;C. Kang
我们以2.4 Å的分辨率解析了人类线粒体的同四聚体单链DNA结合（SSB）蛋白的晶体结构。该四聚体是由两个二聚体头对头相互作用形成的，呈现D2对称。序列相关的四聚体SSB蛋白存在于原核生物和真核生物的线粒体中；这是首次报道这类蛋白质的原子分辨率结构。利用生化数据和序列同源性分析，我们能够将功能特性与结构联系起来。我们提出ssDNA通过柔性环引导的正电通道包裹在四聚体HsmtSSB蛋白上。
We solved the crystal structure of the homotetrameric single-stranded DNA binding (SSB) protein from human mitochondria at a resoluntion of 2.4 Å. The tetramer is formed by two dimers interacting head-to-head and shows D2 symmetry. Sequence-related tetrameric SSB proteins occur in prokaryotes and eukaryotic mitochondria; this is the first report of an atomic resolution structure of this type of protein. Using biochemical data and analysis of sequence homologies, we were able to correlate the functional properties with structure. We propose that ssDNA wraps around the tetrameric HsmtSSB protein through electropositive channels guided by flexible loops.
影响因子：
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作者：
B. C. Wang
通讯作者：
B. C. Wang
大肠杆菌单链 DNA 结合蛋白与寡脱氧核苷酸的光化学交联。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Merrill,BM;Williams,KR;Chase,JW;Konigsberg,WH
通讯作者：
Konigsberg,WH