Brown adipose tissue sympathetic nerve activity is potentiated by activation of 5-hydroxytryptamine (5-HT)1A/5-HT7 receptors in the rat spinal cord.
Brown adipose tissue sympathetic nerve activity is potentiated by activation of 5-hydroxytryptamine (5-HT)1A/5-HT7 receptors in the rat spinal cord.
复制标题
大鼠脊髓中 5-羟色胺 (5-HT)1A/5-HT7 受体的激活可增强棕色脂肪组织交感神经活动。
DOI：
10.1016/j.neuropharm.2007.10.019
复制
发表时间：
2008
影响因子：
4.7
通讯作者：
Morrison,SF
中科院分区：
文献类型：
--
作者：
Madden,CJ;Morrison,SF
In urethane-chloralose anesthetized, neuromuscularly blocked, ventilated rats, microinjection of NMDA (12pmol) into the right fourth thoracic segment (T4) spinal intermediolateral nucleus (IML) immediately increased ipsilateral brown adipose tissue (BAT) sympathetic nerve activity (SNA; peak +492% of control), expired CO2(+0.1%) heart rate (+48beatsmin−1) and arterial pressure (+8mmHg). The increase in BAT SNA evoked by T4 IML microinjection of NMDA was potentiated when it was administered immediately following a T4 IML microinjection of 5-hydroxytryptamine (5-HT, 100pmol) or the 5-HT1A/5-HT7receptor agonist, 8-OH-DPAT (600pmol), (area under the curve: 184%, and 259% of the NMDA-only response, respectively). In contrast, T4 IML microinjection of the 5-HT2receptor agonist, DOI (28pmol) did not potentiate the NMDA-evoked increase in BAT SNA (101% of NMDA-only response). Microinjection into the T4 IML of the selective 5-HT1Aantagonist, WAY-100635 (500pmol), plus the 5-HT7antagonist, SB-269970 (500pmol), prevented the 5-HT-induced potentiation of the NMDA-evoked increase in BAT SNA. When administered separately, WAY-100635 (800pmol) and SB-269970 (800pmol) attenuated the 8-OH-DPAT-induced potentiation of the NMDA-evoked increase in BAT SNA through effects on the amplitude and duration of the response, respectively. The selective 5-HT2receptor antagonist, ketanserin (100pmol), did not attenuate the potentiations of the NMDA-evoked increase in BAT SNA induced by either 5-HT or 8-OH-DPAT. These results demonstrate that activation of 5-HT1A/5-HT7receptors can act synergistically with NMDA receptor activation within the IML to markedly increase BAT SNA.
