Tear Fluid Protein Changes in Dry Eye Syndrome Associated with Rheumatoid Arthritis: A Proteomic Approach
Tear Fluid Protein Changes in Dry Eye Syndrome Associated with Rheumatoid Arthritis: A Proteomic Approach
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DOI：
10.1016/j.jtos.2016.09.005
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发表时间：
2017-01-01
期刊：
影响因子：
6.4
通讯作者：
Angayarkanni, Narayanasamy
中科院分区：
文献类型：
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作者：
Aluru, Saijyothi Venkata;Shweta, Agarwal;Angayarkanni, Narayanasamy
目的：类风湿关节炎（RA）继发的Sjogren综合征（SS）会影响泪腺和唾液腺，因此在RA患者中，干眼综合征（DES）更为普遍。这项研究使用蛋白质组学方法来鉴定继发于RA（DES-RA）的DES撕裂中的潜在生物标志物。方法：用DES患者，患有其他类型的干眼症患者（即原发性Sjogrens和非官方[NSS]）和年龄匹配的对照组收集撕裂标本。对泪蛋白进行2D差异凝胶电泳（2D-DIGE），并使用Nano ESI-LC-MS/ MS分析鉴定出差异表达的蛋白质。结果：在鉴定出鉴定的DES-RA的差异调节蛋白中，发现在100％病例中发现乳酸替代蛋白1的前体1在63％的病例中下调1同工型，而蛋白质（如核糖核酸中）P蛋白p蛋白亚基等蛋白质。在超过80％的病例中，20，原钙粘蛋白和异质核核糖核蛋白Q同工蛋白6被下调。在80％的病例中，将蛋白质（例如ECTO-ADP核糖基转移酶5前体，与RHO相关的GTP结合蛋白和RhoJ前体）上调。结论：功能注释表明，这些蛋白质在调节，抗菌活性，免疫，代谢和细胞过程中具有作用。该研究观察到以前未报告的DES-RA中差异表达的特征标记蛋白。需要进一步验证。
Purpose: Sjogren syndrome (SS) secondary to rheumatoid arthritis (RA) affects lacrimal and salivary glands, and therefore dry eye syndrome (DES) is more prevalent in patients with RA. This study used a proteomic approach to identify potential biomarkers in tear of DES secondary to RA (DES-RA). Methods: Tear specimens were collected with Schirmer strips from patients with DES with RA, patients with other types of dry eye (namely, primary Sjogrens and non-Sjogrens [NSS]), and age-matched controls. Tear proteins were subjected to 2D-differential gel electrophoresis (2D-DIGE), and the differentially expressed proteins were identified using nano ESI-LC-MS/ MS analysis. Results: Among the differentially regulated proteins of DES-RA that were identified, lactotransferrin isoform 1 precursor was found to be down-regulated in 100% cases and SHC transforming 1 isoform in 63% of the cases, while proteins such as ribonuclease p protein subunit 20, protocadherin, and heterogeneous nuclear ribonucleoprotein Q isoform 6 were down-regulated in over 80% of the cases. Proteins such as Ecto-ADP ribosyltransferase 5 precursor, Rho-related GTP-binding protein, and RhoJ precursor were up-regulated in 80% of the cases. Conclusion: Functional annotation revealed that these proteins have roles in regulation, antimicrobial activity, immune, metabolic, and cellular processes. The study observed characteristic marker proteins differentially expressed in DES-RA that are previously unreported. Further validation is needed.