The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death
The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death
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拟南芥LSD1基因在调控低温依赖性细胞死亡中发挥重要作用
DOI：
10.1111/j.1469-8137.2010.03275.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Yang, Shuhua
中科院分区：
文献类型：
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作者：
Huang, Xiaozhen;Li, Yansha;Yang, Shuhua
对两个低温敏感突变体chs 4 -1和chs 4 -3进行了遗传和分子生物学研究，通过图位克隆的方法，发现CHS 4基因与LSD 1基因完全一致，并与LSD 1基因进行了同源性分析。因此，我们将这两个等位基因分别重命名为lsd 1 -3和lsd 1 -4。这两个突变体在冷胁迫条件下表现出广泛的细胞死亡表型。结果表明，lsd 1 -3植株在低温胁迫下，PR 1和PR 2基因表达上调，水杨酸积累增加。这些结果表明低温是lsd 1突变体细胞死亡的另一个触发因素。此外，lsd 1 -3植物积累了较高浓度的过氧化氢和总谷胱甘肽在寒冷条件下比野生型植物。遗传分析表明，LSD 1 - 3的低温敏感表型需要两个关键的信号调节因子PAD 4和EDS 1介导的抗性反应。这些结果揭示了LSD 1在低温胁迫下的细胞死亡调控中的作用，以及低温胁迫反应与ROS相关信号之间的联系。
P>In higher plants, the crosstalk between cold stress responses and reactive oxygen species (ROS) signaling is not well understood.Two chilling-sensitive mutants, chs4-1 and chs4-3, were characterized genetically and molecularly.The CHS4 gene, identified by map-based cloning, was found to be identical to LESION SIMULATING DISEASE RESISTANCE 1 (LSD1). We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid. These results indicate that low temperature is another trigger of cell death in lsd1 mutants. Furthermore, lsd1-3 plants accumulated higher concentrations of H2O2 and total glutathione under cold conditions than wild-type plants. Genetic analysis revealed that PAD4 and EDS1, two key signaling regulators mediating resistance responses, are required for the chilling-sensitive phenotype of lsd1-3.These findings reveal a role of LSD1 in regulating cell death trigged by cold stress and a link between cold stress responses and ROS-associated signaling.