Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer.
Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer.
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循环外泌体 microRNA 作为非小细胞肺癌的预后生物标志物
DOI：
10.18632/oncotarget.14369
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发表时间：
2017-02-21
期刊：
影响因子：
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通讯作者：
Li Y
中科院分区：
文献类型：
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作者：
Liu Q;Yu Z;Yuan S;Xie W;Li C;Hu Z;Xiang Y;Wu N;Wu L;Bai L;Li Y
外体miRNAs被认为是临床应用的良好候选生物标记物。然而，人们对它们作为肺癌预后生物标志物的潜在作用知之甚少。在这项研究中，我们探讨了血浆外体microRNAs(MiRNAs)对非小细胞肺癌(NSCLC)的预后价值。应用定量聚合酶链式反应(QPCR)芯片分析了10例肺腺癌患者和10例健康对照的84个外体miRNAs。QPCR阵列显示30个异常表达的外体miRNAs。根据差异表达和以前的报道，选择了9个候选miRNAs，以进一步评估它们在196例NSCLC患者中的预后作用。外体miR-23b-3p、miR-10b-5p和miR-21-5p水平升高与总体生存不良独立相关(风险比[95%可信区间]分别为2.42(1.45~4.04)，P=0.001；2.22(1.18~4.16)，P=0.013；2.12(1.28~3.49)，P=0.003)。与单纯临床预后变量模型相比，三个外体miRNA信号的加入显著提高了生存预测的准确性，受试者操作特征曲线下的时间依赖面积从0.88增加到0.91(P=0.015)。提示血浆外体miR-23b-3p、miR-10b-5p和miR-21-5p可作为非小细胞肺癌的无创预后标志物。
Exosomal miRNAs are proposed as excellent candidate biomarkers for clinical applications. However, little is known about their potential roles as prognostic biomarkers in lung cancer. In this study, we explored the prognostic value of plasma exosomal microRNAs (miRNAs) for non-small-cell lung cancer (NSCLC). Using a quantitative polymerase chain reaction (qPCR) array panel, we analyzed 84 plasma exosomal miRNAs in 10 lung adenocarcinoma patients and 10 matched healthy controls. The qPCR array showed 30 aberrantly expressed exosomal miRNAs. Nine candidate miRNAs were selected based on differential expression and previous reports for further evaluating their prognostic roles in 196 NSCLC patients. Elevated levels of exosomal miR-23b-3p, miR-10b-5p and miR-21-5p were independently associated with poor overall survival (with hazard ratio [95% confidence interval]: 2.42 (1.45 - 4.04), P = 0.001; 2.22 (1.18 - 4.16), P = 0.013; 2.12 (1.28 - 3.49), P = 0.003, respectively). When compared to the clinical prognostic variables only model, adding the three exosomal miRNA signatures significantly improved survival predictive accuracy with an increase of time-dependent area under the receiver operating characteristic curve from 0.88 to 0.91 (P=0.015). Our results indicated that plasma exosomal miR-23b-3p, miR-10b-5p and miR-21-5p are promising non-invasive prognostic biomarkers of NSCLC.