Sequence and functional divergence of gametocyte-specific parasitophorous vacuole membrane proteins in Plasmodium parasites
Sequence and functional divergence of gametocyte-specific parasitophorous vacuole membrane proteins in Plasmodium parasites
复制标题
DOI：
10.1016/j.molbiopara.2018.01.002
复制
发表时间：
2018-03-01
影响因子：
1.5
通讯作者：
Siden-Kiamos, Inga
中科院分区：
文献类型：
--
作者：
Deligianni, Elena;Andreadaki, Maria;Siden-Kiamos, Inga
疟原虫在红细胞内发育，在一个被PVM膜包裹的寄生空泡中。蛋白家族ETRAMP(早期转录膜蛋白)由通过单一跨膜区插入PVM的小蛋白组成。其中，Pfs16基因在恶性疟原虫配子体PVM中有特异性表达。伯氏疟原虫PBANKA_1003900基因与pfs16同源。编码的蛋白质具有相似的结构域结构，但总体上蛋白质相似性较低。Berghei基因的转录本只在配子细胞和动子中发现，该蛋白的C端mCherry融合表明它只存在于配子细胞中。PBANKA_1003900基因敲除突变体在性发育过程中没有受到影响，动粒的形成与WT相似。突变对通过蚊子传播没有不良影响，尽管突变寄生虫形成的卵囊数量减少了。
Plasmodium parasites develop within red blood cells in a Parasitophorous Vacuole enclosed by a Membrane, the PVM. The protein family ETRAMP (Early Transcribed Membrane Protein) comprises small proteins inserted in the PVM via a single transmembrane domain. Among those, Pfs16 is specifically found in P. falciparum gametocyte PVM. The P. berghei gene PBANKA_1003900 is syntenic with pfs16. The encoded proteins have a similar domain structure but the overall protein similarity is low. A transcript of the P. berghei gene is only found in gametocytes and ookinetes and a C-terminal mCherry fusion of the protein revealed its presence only in gametocytes. A knock-out mutant of the PBANKA_1003900 gene was not affected in sexual development and ookinete formation was similar to WT. The mutation had no adverse effect on transmission through the mosquito although there was a reduction of the number of oocysts formed by the mutant parasites