The rice CONSTANS-like protein OsCOL15 suppresses flowering by promoting Ghd7 and repressing RID1.
The rice CONSTANS-like protein OsCOL15 suppresses flowering by promoting Ghd7 and repressing RID1.
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DOI：
10.1016/j.bbrc.2017.11.095
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发表时间：
2018
影响因子：
3.1
通讯作者：
Weixun Wu;Ying-xin Zhang;Miao Zhang;X. Zhan;Xihong Shen;P. Yu;Daibo B. Chen;Qunen Liu;Sittipun Sinumporn;K. Hussain;Shihua Cheng;Liyong Cao
中科院分区：
文献类型：
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作者：
Weixun Wu;Ying-xin Zhang;Miao Zhang;X. Zhan;Xihong Shen;P. Yu;Daibo B. Chen;Qunen Liu;Sittipun Sinumporn;K. Hussain;Shihua Cheng;Liyong Cao
The photoperiodic flowering pathway is one of the most important regulatory networks controlling flowering time in rice (Oryza sativaL.). Rice is a facultative short-day (SD) plant; flowering is promoted under inductive SD conditions and delayed under non-inductive long-day (LD) conditions. In rice, flowering inhibitor genes play an important role in maintaining the trade-off between reproduction and yield. In this study, we identified a novel floral inhibitor,OsCOL15, which encodes a CONSTANS-like transcription factor. Consistent with a function in transcriptional regulation, OsCOL15 localized to the nucleus. Moreover, OsCOL15 had transcriptional activation activity, and the central region of the protein between the B-box and CCT domains was required for this activity. We determined thatOsCOL15is most highly expressed in young organs and exhibits a diurnal expression pattern typical of other floral regulators. Overexpression ofOsCOL15resulted in a delayed flowering phenotype under both SD and LD conditions. Real-time quantitative RT-PCR analysis of flowering regulator gene expression suggested thatOsCOL15suppresses flowering by up-regulating the flowering repressorGrain number,plant heightandheading date 7(Ghd7) and down-regulating the flowering activatorRice Indeterminate 1(RID1), thus leading to the down-regulation of the flowering activatorsEarly heading date 1,Heading date 3a, andRICE FLOWERING LOCUS T1. These results demonstrate thatOsCOL15is an important floral regulator acting upstream ofGhd7andRID1in the rice photoperiodic flowering-time regulatory network.
