Hepatitis B virus X gene can transactivate heterologous viral sequences.
Hepatitis B virus X gene can transactivate heterologous viral sequences.
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乙型肝炎病毒X基因可以反式激活异源病毒序列。
DOI：
10.1073/pnas.86.6.2046
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发表时间：
1989
影响因子：
11.1
通讯作者：
Robinson,WS
中科院分区：
文献类型：
--
作者：
Twu,JS;Robinson,WS
B型肝炎病毒（HBV）最小的开放阅读框被命名为X基因，其在HBV感染和复制过程中的生物学功能尚不清楚。本文所述的实验表明，在含有氯霉素乙酰转移酶（CAT）基因的质粒的HepG 2细胞中HBV X基因的表达在人类免疫缺陷病毒（HIV-1）长末端重复序列（LTR）的控制下，导致CAT基因转录以及基因产物（CAT）的表达显著增加。HIV-1 tatIII基因和HBV X基因一起增加HIV-1 LTR调节的CAT表达，高于单独使用任一基因观察到的水平，表明X基因和达特的协同作用。HBV X基因也刺激CAT基因在猴病毒40增强子和早期启动子控制下的表达，但不刺激visna病毒LTR或人T细胞嗜淋巴细胞病毒I型（HTLV-I）LTR，表明HBV X基因可以反式激活某些而不是其他异源病毒序列。HBV X基因对HIV-1 LTR的反式激活在不同的细胞系中不同，这表明它可能是由细胞因子介导的。
The smallest open reading frame of hepatitis B virus (HBV) has been designated the X gene and its biological function during HBV infection and replication is not known. Experiments described here demonstrate that expression of the HBV X gene in HepG2 cells containing a plasmid with the chloramphenicol acetyltransferase (CAT) gene under control of the human immunodeficiency virus (HIV-1) long terminal repeat (LTR) sequence leads to a marked increase in CAT gene transcription as well as expression of the gene product (CAT). The HIV-1 tatIII gene and the HBV X gene together increased HIV-1 LTR-regulated CAT expression above that observed with either gene alone, suggesting a synergistic effect of the X gene and tat. HBV X gene also stimulated expression of the CAT gene under control of the simian virus 40 enhancer and early promoter but not the visna virus LTR or the human T-cell lymphotropic virus type I (HTLV-I) LTR, indicating that the HBV X gene can transactivate some but not other heterologous viral sequences. Transactivation of the HIV-1 LTR by the HBV X gene varied in different cell lines, suggesting that it may be mediated by a cellular factor(s).