Colistin resistance prevalence in Escherichia coli from domestic animals in intensive breeding farms of Jiangsu Province
Colistin resistance prevalence in Escherichia coli from domestic animals in intensive breeding farms of Jiangsu Province
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江苏省集约化养殖场家畜大肠杆菌粘菌素耐药情况
DOI：
10.1016/j.ijfoodmicro.2018.11.013
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发表时间：
2019-02-16
影响因子：
5.4
通讯作者：
He, K.
中科院分区：
文献类型：
--
作者：
Zhang, X.;Zhang, B.;He, K.
粘菌素耐药性的全球传播受到了极大的关注。近年来，中国地区动物、食品和患者分离的大肠埃希菌对mcr基因编码的质粒介导的粘菌素耐药性报道较多。为了解江苏省家畜对粘菌素的耐药性及mcr-1和mcr-2基因在家畜中的传播情况，我们采集了集约化养殖场不同日龄的猪、鸡和牛的粪便拭子。用选择性显色琼脂和mcr-PCR方法筛选粘菌素抗性和mcr基因携带者。猪、鸡、牛粪便标本中粘菌素耐药菌落数分别为54.25%(440/811)、35.96%(443/1232)和26.92%(42/156)。在猪、鸡和牛分离的粘菌素耐药株中，mcr-1的阳性率分别为68.86%(303/440)、87.58%(388/443)和71.43%(30/42)，而mcr-2在猪、鸡和牛中的阳性率分别为46.82%(206/440)、14.90%(66/443)和19.05%(8/42)。Mcr-1和mcr-2在猪、鸡和牛中共存的比例分别为20%(88/440)、7.22%(32/443)和9.52%(4/42)。有必要采取干预措施和替代选择，以最大限度地减少mcr在食用动物和人类之间的进一步传播。
The global dissemination of colistin resistance has received a great deal of attention. Recently, the plasmid-mediated colistin resistance encoded by mcr genes in Escherichia coli (E. coil) strains from animals, food, and patients in China has been reported frequently. To investigate the colistin resistance and mcr-1 and mcr-2 genes spread in domestic animals in Jiangsu Province, we collected fecael swabs from pigs, chicken and cattle at different ages distributed in intensive feeding farms. The selective chromogenic agar and mcr-PCR were used to screen the colistin resistance and mcr gene carriage. Colistin resistant E. coli colonies were identified in 54.25% (440/811), 35.96% (443/1232), and 26.92% (42/156) faecal swabs from pigs, chickens, and cattle, respectively. The prevalence of mcr-1 in colistin resistant E. coli isolates from pigs, chickens and cattle was 68.86% (303/440), 87.58% (388/443), and 71.43% (30/42), respectively, compared to mcr-2 which was present in 46.82% (206/440), 14.90% (66/443), and 19.05% (8/42) of the colistin-resistant E. coli isolated from pigs, chickens and cattle, respectively. Co-occurrence of mcr-1 and mcr-2 was identified in 20% (88/440) in pigs, 7.22% (32/443) in chickens, and 9.52% (4/42) in cattle. Interventions and alternative options are necessary to minimise further dissemination of mcr between food-producing animals and human.