A meta-analysis of the microbial diversity observed in anaerobic digesters
A meta-analysis of the microbial diversity observed in anaerobic digesters
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DOI：
10.1016/j.biortech.2010.11.119
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发表时间：
2011-02-01
影响因子：
11.4
通讯作者：
Yu, Zhongtang
中科院分区：
文献类型：
--
作者：
Nelson, Michael C.;Morrison, Mark;Yu, Zhongtang
在这项研究中，集体微生物多样性的厌氧消化池进行了检查，使用荟萃分析方法。所有16S rRNA基因序列回收厌氧消化器在公共数据库中检索和系统发育和统计分析。截至2010年5月，在GenBank中发现了16，519个细菌和2869个古细菌序列。细菌序列被分配到代表28个已知细菌门的5926个操作分类单位（OTU，基于>= 97%序列同一性），其中变形菌门（1590个OTU）、厚壁菌门（1352个OTU）、拟杆菌门（705个OTU）和绿杆菌门（693个OTU）是主要的。将序列分配给296个OTU，主要是Methanosaeta和未表征的WSA 2组。近60%的序列无法归类于任何已确定的属。稀疏分析表明，在厌氧消化器中的细菌和古菌的多样性的约60%和90%已被采样。AD系统中全球细菌和古菌多样性的分析可以指导未来的研究，以进一步研究AD中涉及的微生物多样性和开发全面的分析工具。(C)2010爱思唯尔有限公司版权所有。
In this study, the collective microbial diversity in anaerobic digesters was examined using a meta-analysis approach. All 16S rRNA gene sequences recovered from anaerobic digesters available in public databases were retrieved and subjected to phylogenetic and statistical analyses. As of May 2010, 16,519 bacterial and 2869 archaeal sequences were found in GenBank. The bacterial sequences were assigned to 5926 operational taxonomic units (OTUs, based on >= 97% sequence identity) representing 28 known bacterial phyla, with Proteobacteria (1590 OTUs), Firmicutes (1352 OTUs), Bacteroidetes (705 OTUs), and Chloroflexi (693 OTUs) being predominant. Archaeal sequences were assigned to 296 OTUs, primarily Methanosaeta and the uncharacterized WSA2 group. Nearly 60% of all sequences could not be classified to any established genus. Rarefaction analysis indicates that approximately 60% of bacterial and 90% of archaeal diversity in anaerobic digesters has been sampled. This analysis of the global bacterial and archaeal diversity in AD systems can guide future studies to further examine the microbial diversity involved in AD and development of comprehensive analytical tools. (C) 2010 Elsevier Ltd. All rights reserved.