Nocardia jejuensis sp nov., a novel actinomycete isolated from a natural cave on Jeju Island, Republic of Korea
Nocardia jejuensis sp nov., a novel actinomycete isolated from a natural cave on Jeju Island, Republic of Korea
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DOI：
10.1099/ijs.0.63866-0
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发表时间：
2006-03-01
影响因子：
2.8
通讯作者：
Lee, SD
中科院分区：
文献类型：
--
作者：
Lee, SD
用稀释法从韩国济州岛的一个天然洞穴中分离到一株新的放线菌N3-2(T)，并进行了多相分类。通过对纯化的PCR产物进行直接测序，确定了几乎完整的16S rRNA基因序列，并将其与诺卡氏菌属代表的序列进行了比较。系统发育分析表明，在诺卡氏菌科诺卡氏菌属的进化半径内，该菌在沙门诺卡氏菌群和白色诺卡氏菌分支之间形成了一个明显的分支。该菌的16S rRNA基因序列与其最近的系统发育近邻，即沙门氏菌和白纹伊蚊的相似性为97-4%。化学分类特征，如主要氨基酸的肽聚糖、主要的菜籽酮和极性脂，支持诺卡氏菌属的分类。通过广泛的表型特性和其独特的系统发育地位，该生物很容易与有效公布的名称的诺卡氏菌物种区分开来；名称为诺卡氏菌jjuensis sp.11月N3-2(T)(=JCM 13281(T)=NRRL B-24430(T))为模式菌株。
A novel actinomycete, strain N3-2(T), was isolated from a natural cave on Jeju Island, Republic of Korea, using a dilution method and was subjected to polyphasic taxonomy. The almost complete 16S rRNA gene sequence was determined by direct sequencing of the purified PCR product and was compared with those of representatives of the genus Nocardia. It was revealed from the phylogenetic analysis that the organism forms a distinct clade between the Nocardia salmonicida cluster and the Nocardia alba branch within the evolutionary radius occupied by the genus Nocardia of the family Nocardiaceae. The organism showed 16S rRNA gene sequence similarity of 97-4% with its nearest phylogenetic neighbours, namely N. salmonicida and N. alba. The chemotaxonomic properties, such as the principal amino acid of peptidoglycan, predominant menaquinone and polar lipids, supported the classification in the genus Nocardia. The organism was readily differentiated from Nocardia species with validly published names by a broad set of phenotypic properties and its unique phylogenetic position; the name Nocardia jejuensis sp. nov. is proposed, with N3-2(T) (=JCM 13281(T)=NRRL B-24430(T)) as the type strain.