Respective roles of organic and mineral components of human cortical bone matrix in micromechanical behavior: An instrumented indentation study
Respective roles of organic and mineral components of human cortical bone matrix in micromechanical behavior: An instrumented indentation study
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DOI：
10.1016/j.jmbbm.2011.05.017
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发表时间：
2011-10-01
影响因子：
3.9
通讯作者：
Boivin, G.
中科院分区：
文献类型：
--
作者：
Bala, Y.;Depalle, B.;Boivin, G.
骨是由I型胶原基质和结晶较差的磷灰石矿物相组成的多尺度复合材料。由于重塑活动，皮质骨由称为骨单位的骨结构单位（BSU）组成。由于骨单位代表了结构层次的一个基本水平，因此研究该尺度下的力学行为与组织组成之间的关系对于更好地理解骨脆性的机制是非常重要的。本研究的目的是分析骨组织的超微结构特性和力学行为之间的联系，在骨单位的规模。髂骨活检取自未经治疗的绝经后骨质疏松症的妇女，包埋，切片和显微X线摄影，以评估骨矿化度（DMB）。在每个切片上，用相对高的载荷（类似于500 mN）压入已知DMB的BSU，以确定几个骨板的局部弹性模量（E）、接触硬度（H-c）和真实硬度（H）。通过傅里叶变换红外显微光谱法（FTIRM）测量相同BSU的结晶度和胶原成熟度。采用多元回归分析方法分析了力学性能与超微结构成分之间的相互关系，结果表明弹性变形仅由DMB解释，而塑性变形与胶原成熟度的相关性更大。接触硬度反映了弹性和塑性行为，与DMB和胶原成熟度相关。在骨单位水平上，结晶度和力学性能之间没有关系。(C)2011爱思唯尔有限公司保留所有权利。
Bone is a multiscale composite material made of both a type I collagen matrix and a poorly crystalline apatite mineral phase. Due to remodeling activity, cortical bone is made of Bone Structural Units (BSUs) called osteons. Since osteon represents a fundamental level of structural hierarchy, it is important to investigate the relationship between mechanical behavior and tissue composition at this scale for a better understanding of the mechanisms of bone fragility. The aim of this study is to analyze the links between ultrastructural properties and the mechanical behavior of bone tissue at the scale of osteon.Iliac bone biopsies were taken from untreated postmenopausal osteoporotic women, embedded, sectioned and microradiographed to assess the degree of mineralization of bone (DMB). On each section, BSUs of known DMB were indented with relatively high load (similar to 500 mN) to determine local elastic modulus (E), contact hardness (H-c) and true hardness (H) of several bone lamellae. Crystallinity and collagen maturity were measured by Fourier Transform InfraRed Microspectroscopy (FTIRM) on the same BSUs. Inter-relationships between mechanical properties and ultrastructural components were analyzed using multiple regression analysis.This study showed that elastic deformation was only explained by DMB whereas plastic deformation was more correlated with collagen maturity. Contact hardness, reflecting both elastic and plastic behaviors, was correlated with both DMB and collagen maturity. No relationship was found between crystallinity and mechanical properties at the osteon level. (C) 2011 Elsevier Ltd. All rights reserved.