Fluorescent Gold Nanoclusters for In Vivo Shortwave-Infrared Imaging
Fluorescent Gold Nanoclusters for In Vivo Shortwave-Infrared Imaging
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DOI：
10.1149/2162-8777/ac258c
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发表时间：
2021-09
影响因子：
2.2
通讯作者：
Setsuko Tsuboi;T. Jin
中科院分区：
文献类型：
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作者：
Setsuko Tsuboi;T. Jin
金纳米团簇（Au-NC）是一种独特的荧光纳米材料，可用于体外和体内生物成像。Au-NC毒性较小，其尺寸比其他荧光纳米材料（如量子点）小得多。Au-NC的发射波长取决于纳米团簇的尺寸，这可以通过反应条件来控制。最近，短波红外（SWIR）发射的金纳米粒子的生物成像引起了广泛的关注。SWIR荧光（900-1400 nm）由于SWIR荧光被组织的弱吸收和散射而使得能够进行深层组织成像。由于组织的自发荧光在SWIR区域中几乎为零，因此与可见光和近红外成像相比，SWIR荧光成像导致高对比度图像。对于短波红外荧光成像，生物相容性短波红外荧光探针的开发是至关重要的。虽然已经出现了基于量子点、稀土掺杂纳米颗粒和有机染料的几种类型的SWIR荧光探针，但是很少有SWIR探针的细胞毒性低到足以用于生物医学领域。在此，我们提出了牛血清白蛋白（BSA）的冠状金纳米粒子（Au-NCs@ BSA），在短波红外区发射的合成，和光学性质。我们证明了Au-NCs@ BSA用于小鼠脑血管、血管、淋巴结和肿瘤的体内SWIR荧光成像的功效。
Gold nanoclusters (Au-NCs) are unique fluorescent nanomaterials that can be used for in vitro and in vivo bioimaging. Au-NCs are less toxic, and their size is much smaller than that of other fluorescent nanomaterials such as quantum dots. The emission wavelengths of Au-NCs depend on the nanocluster size, which can be controlled by the reaction conditions. Recently, shortwave-infrared (SWIR) emitting Au-NCs have attracted much attention for bioimaging. SWIR fluorescence (900–1400 nm) enables deep tissue imaging due to the weak absorption and scattering of the SWIR fluorescence by tissues. Since the autofluorescence of tissues is almost zero in the SWIR region, SWIR fluorescence imaging results in high contrast images compared to visible and near-infrared imaging. For the SWIR fluorescence imaging, the development of biocompatible SWIR fluorescent probes is crucial. Although several types of SWIR fluorescent probes based on quantum dots, rare-earth-doped nanoparticles, and organic dyes have been appeared, there are very few examples of SWIR probe which cytotoxicity is low enough to use in biomedical fields. Herein, we present the synthesis, and optical properties of bovine serum albumin (BSA) capped Au-NCs (Au-NCs@ BSA), which emit at the SWIR region. We demonstrate the efficacy of Au-NCs@ BSA for in vivo SWIR fluorescence imaging of brain vasculatures, blood vessels, lymph nodes and tumors in mice.