Mutations affecting eye morphology in the developing zebrafish (Danio rerio)
Mutations affecting eye morphology in the developing zebrafish (Danio rerio)
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DOI：
10.1002/(sici)1520-6408(1997)20:3
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发表时间：
1997-01-01
期刊：
影响因子：
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通讯作者：
Dowling, JE
中科院分区：
文献类型：
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作者：
Fadool, JM;Brockerhoff, SE;Dowling, JE
斑马鱼（Danio rerio）作为脊椎动物发育的分子和遗传研究的主流模式动物，受到了相当大的关注。在我们的实验室中，我们进行了第三代化学诱变斑马鱼的隐性突变影响视觉系统的屏幕。本报告描述了可见的表型和数量的形态突变体，迄今观察到的，并提出了更详细的组织学分析，这些突变。通过对突变幼虫的分析，确定了几种细微的形态学突变导致视网膜特定细胞层的变性。其他突变导致一些变性弥漫性分布在整个视网膜或集中在视网膜边缘。影响视杯内陷和透镜囊泡形成的单一突变导致前房形成失败。这些结果证明了小规模、高度聚焦的屏幕用于揭示涉及视网膜和眼睛发育的新位点的实用性。(C)1997 Wiley-Liss，Inc.
The zebrafish (Danio rerio) has received considerable attention as a mainstream model for the molecular and genetic study of vertebrate development. In our laboratory, we have conducted a third-generation screen of chemically mutagenized zebrafish for recessive mutations affecting the visual system. This report describes the visible phenotypes and number of morphological mutants so far observed and presents a more detailed histological analysis of six of these mutations. Through analysis of mutant larvae, it was determined that several of the subtle morphological mutations resulted in degeneration of specific cellular layers of he retina. Other mutations resulted in some degeneration distributed diffusely across the entire retina or concentrated at the retinal margin. A single mutation affecting invagination of the optic cup and lens vesicle Formation resulted in a failure to develop an anterior chamber. These results demonstrate the utility of a small-scale, highly focused screen for uncovering novel loci involved in retinal and eye development. (C) 1997 Wiley-Liss, Inc.