Bioinspired 3D microprinted cell scaffolds: Integration of graph theory to recapitulate complex network wiring in lymph nodes.
Bioinspired 3D microprinted cell scaffolds: Integration of graph theory to recapitulate complex network wiring in lymph nodes.
复制标题
仿生 3D 微打印细胞支架：整合图论来概括淋巴结中复杂的网络布线。
DOI：
10.1002/biot.202300359
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发表时间：
2024
影响因子：
4.7
通讯作者：
Coppens,Marc-Olivier
中科院分区：
文献类型：
--
作者：
Chin,MatthewHW;Reid,Barry;Lachina,Veronika;Acton,SophieE;Coppens,Marc-Olivier
物理网络在自然界中无处不在，但其中许多网络具有复杂的组织结构，难以在人工系统中重现。在生物医学和组织工程中尤其如此，其中3D细胞支架的微观结构细节非常重要。生物网络的研究，如成纤维细胞网状细胞（FRC）网络，揭示了网络拓扑结构在一系列生物功能中的关键作用。然而，细胞支架很少使用图论进行分析或设计。为了了解网络如何影响粘附的细胞，需要捕获生物相关网络的复杂拓扑特性的3D培养平台。在这项工作中，我们从FRC网络的小世界组织（高聚类和低路径长度）中获得灵感来设计细胞支架。创建了一个算法工具集来生成网络并对其进行处理，以提高其3D打印性能。我们使用图论的工具来证明网络是小世界的（Ω因子，ω=-0.10 ± 0.02;小世界倾向，SWP = 0.74 ± 0.01）。采用3D微打印将网络物理化为支架，这支持了FRC的存活。因此，这项工作代表了一种生物启发的、图论驱动的方法来控制微尺度细胞生态位网络。
Physical networks are ubiquitous in nature, but many of them possess a complex organizational structure that is difficult to recapitulate in artificial systems. This is especially the case in biomedical and tissue engineering, where the microstructural details of 3D cell scaffolds are important. Studies of biological networks—such as fibroblastic reticular cell (FRC) networks—have revealed the crucial role of network topology in a range of biological functions. However, cell scaffolds are rarely analyzed, or designed, using graph theory. To understand how networks affect adhered cells, 3D culture platforms capturing the complex topological properties of biologically relevant networks would be needed. In this work, we took inspiration from the small‐world organization (high clustering and low path length) of FRC networks to design cell scaffolds. An algorithmic toolset was created to generate the networks and process them to improve their 3D printability. We employed tools from graph theory to show that the networks were small‐world (omega factor,ω= ‐0.10 ± 0.02; small‐world propensity, SWP = 0.74 ± 0.01). 3D microprinting was employed to physicalize networks as scaffolds, which supported the survival of FRCs. This work, therefore, represents a bioinspired, graph theory‐driven approach to control the networks of microscale cell niches.