Ethylene biosynthesis and endogenous polyamines in relation to development of in vitro cultured kiwifruit explants.
Ethylene biosynthesis and endogenous polyamines in relation to development of in vitro cultured kiwifruit explants.
复制标题
乙烯生物合成和内源多胺与体外培养的猕猴桃外植体发育的关系。
DOI：
10.1071/fp03184
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发表时间：
2004
影响因子：
3
通讯作者：
Aída González
中科院分区：
文献类型：
--
作者：
L. Arigita;R. S. Tamés;Aída González
多胺和乙烯之间的关系是有争议的，因为一种途径与另一种途径的干扰程度可能因物种、发育阶段和实验程序而异。本文通过添加氨基乙氧基乙烯基甘氨酸（AVG）或1-氨基环丙烷-1-羧酸（ACC）对乙烯生物合成进行修饰，研究其对猕猴桃外植体（Actinidia deliciosa CS Liang）多胺含量和发育的影响。& AR Fergusson)。在没有乙烯积累的通风条件下培养的猕猴桃外植体ACC合成酶活性高于不通风条件下的猕猴桃外植体，多胺含量低于不通风条件下的猕猴桃外植体。在对照培养基中培养的外植体中，腐胺是含量较高的多胺，而精胺仅在游离部分中检测到，与通风无关。在通风条件下，培养基中添加ACC抑制了器官发生，亚精胺含量减少，且未检测到精胺。在培养基中添加AVG增加了芽数和多胺数量，抑制了ACC合成酶，因此s -腺苷蛋氨酸（SAM）导致亚精胺和精胺的合成增加。在抑制乙烯生物合成的基础上，腐胺的增加更难以解释。由于AVG的加入，猕猴桃外植体的芽数增加，可能是由于容器中大气中乙烯的缺乏或游离多胺的增加。
The relationship between polyamines and ethylene is controversial because the degree of interference of one pathway with the other may differ according to species, stage of development and experimental procedure. In this paper, we modify ethylene biosynthesis by the addition of aminoethoxyvinylglicine (AVG) or 1-aminocyclopropane-1-carboxylic acid (ACC) and study how it affects polyamine content and development of kiwifruit explants (Actinidia deliciosa CS Liang. & AR Fergusson). Cultured under ventilation where ethylene did not accumulate in the culture vessels, kiwi explants had higher ACC synthase activity and lower polyamine content than those grown without ventilation. In explants cultured in the reference medium, putrescine was the more abundant polyamine and spermine was only detected in the free fraction irrespective of ventilation. Under ventilation, addition of ACC to the culture medium inhibited organogenesis, there was less spermidine and spermine was not detected. Addition of AVG to the culture medium increased both the number of shoots and the amount of polyamines, and inhibited ACC synthase, so S-adenosylmethionine (SAM) led to increasing synthesis of spermidine and spermine. The increase in putrescine is more difficult to explain on the basis of inhibition of ethylene biosynthesis. The increase in the number of shoots in kiwi explants due to AVG addition may be attributed to the lack of ethylene in the atmosphere of the vessels or the increase in free polyamines.