Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
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DOI：
10.1073/pnas.160270997
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发表时间：
2000-08-15
影响因子：
11.1
通讯作者：
Chen, JL
中科院分区：
文献类型：
--
作者：
Hu, RM;Han, ZG;Chen, JL
主要的神经内分泌界面，下丘脑和垂体，以及肾上腺，构成了主要的轴，负责维持体内平衡和对环境扰动的反应。通过生成大量表达序列标签（est）对人类下丘脑-垂体-肾上腺轴的基因表达谱进行编目，然后进行生物信息学分析（http://www.chgc.sh.cn/database）。从下丘脑、垂体和肾上腺的cDNA文库中获得31130个克隆（83.4%），共获得25973条高质量序列。在剔除5347条与重复元件和mtDNA对应的序列后，将20626条ESTs整合到9175个簇中（下丘脑、垂体和肾上腺分别为3979、3074和4116个簇）。在这些基因簇中，2777个（30.3%）对应于已知基因，4165个（44.8%）对应于dbest， 2233个（24.3%）对应于新ESTs。基因表达谱很好地反映了下丘脑-垂体-肾上腺轴这三个水平的功能特征，因为20个表达最高的基因在组织分布上大多存在统计学差异，包括一组组织特异性功能标记。同时，在轴的生理学方面取得了一些发现，克隆了200个新基因的全长cdna并进行了测序。所有这些数据可能有助于理解人类生命的神经内分泌调节。
The primary neuroendocrine interface, hypothalamus and pituitary, together with adrenals, constitute the major axis responsible for the maintenance of homeostasis and the response to the perturbations in the environment. The gene expression profiling in the human hypothalamus-pituitary-adrenal axis was catalogued by generating a large amount of expressed sequence tags (ESTs), followed by bioinformatics analysis (http://www.chgc.sh.cn/database). Totally, 25,973 sequences of good quality were obtained from 31,130 clones (83.4%) from cDNA libraries of the hypothalamus, pituitary, and adrenal glands. After eliminating 5,347 sequences corresponding to repetitive elements and mtDNA, 20,626 ESTs could be assembled into 9,175 clusters (3,979, 3,074, and 4,116 clusters in hypothalamus, pituitary, and adrenal glands, respectively) when overlapping ESTs were integrated. Of these clusters, 2,777 (30.3%) corresponded to known genes, 4,165 (44.8%) to dbESTs, and 2,233 (24.3%) to novel ESTs. The gene expression profiles reflected well the functional characteristics of the th ree levels in the hypothalamus-pituitary-adrenal axis, because most of the 20 genes with highest expression showed statistical difference in terms of tissue distribution, including a group of tissue-specific functional markers. Meanwhile, some findings were made with regard to the physiology of the axis, and 200 full-length cDNAs of novel genes were cloned and sequenced. All of these data may contribute to the understanding of the neuroendocrine regulation of human life.