Design and application of a self-pumping microfluidic staggered herringbone mixer
Design and application of a self-pumping microfluidic staggered herringbone mixer
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DOI：
10.1007/s10404-021-02426-x
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发表时间：
2021-03-19
影响因子：
2.8
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
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作者：
Channon, Robert B.;Menger, Ruth F.;Henry, Charles S.
试剂的快速混合对于广泛的化学和生物反应是至关重要的，但是难以在微流体装置中实施，特别是在毛细作用/被动泵送装置中或在需要点的环境中。在这里，我们开发了一种自泵浦非对称交错人字形混频器仅由激光烧蚀玻璃和磁带。该芯片实验室平台能够在没有外力或泵的情况下快速流动（0.14 mL min（-1），1 cm s（-1））和快速混合（< 10 s），并且适合非水溶剂的流动。此外，分别通过人字形凹槽的长度和深度以及限定通道高度的双面胶带的厚度，可以容易地调节混合程度和流速。通过Ni（II）和DMG在乙醇/水中的反应和邻联茴香胺与过氧化物酶的酶促反应，分别证明了该装置的实用性用于化学和生物测定。
The rapid mixing of reagents is critical to a wide range of chemical and biological reactions but is difficult to implement in microfluidic devices, particularly in capillary action/passive pumping devices or in point-of-need environments. Here, we develop a self-pumping asymmetric staggered herringbone mixer made from only laser-ablated glass and tape. This lab-on-a-chip platform is capable of rapid flow (0.14 mL min(-1), 1 cm s(-1)) and fast mixing (< 10 s) without external forces or pumps and is amenable to the flow of non-aqueous solvents. Furthermore, the degree of mixing and flow rates are easily tunable through the length and depth of the herringbone grooves, and the thickness of the double-sided tape that defines the channel height, respectively. The device utility is demonstrated for chemical and biological assays through the reaction of Ni(II) and DMG in ethanol/water and the enzymatic reaction of o-dianisidine with peroxidase, respectively.