SKELETAL-MUSCLE BLOOD-FLOW INDEPENDENTLY MODULATES INSULIN-MEDIATED GLUCOSE-UPTAKE
SKELETAL-MUSCLE BLOOD-FLOW INDEPENDENTLY MODULATES INSULIN-MEDIATED GLUCOSE-UPTAKE
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DOI：
10.1152/ajpendo.1994.266.2.e248
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发表时间：
1994-02-01
影响因子：
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通讯作者：
JOHNSON, A
中科院分区：
文献类型：
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作者：
BARON, AD;STEINBERG, H;JOHNSON, A
胰岛素介导的葡萄糖摄取（IMGU）主要发生在骨骼肌中。为了直接检查骨骼肌灌注（F）是否可以直接和独立地调节IMGU，我们结合了高胰岛素正葡萄糖钳夹和腿部平衡技术[腿部葡萄糖摄取（LGU）=动静脉葡萄糖差异（Δ AVG）× F]。研究了年轻（< 40岁）健康瘦型受试者在血糖正常的高胰岛素血症期间的血糖水平，高胰岛素血症的水平类似于23 μ U/ml（组I，n = 8）和类似于950 μ U/ml（组II，n = 6）。测量股骨Δ AVG和F腿，在类似于单独稳态高胰岛素血症200分钟后以及在叠加股动脉内输注盐酸乙酰甲胆碱期间（旨在使F腿增加2 - 3倍），计算基线时的LGU。在第I组和第II组中，胰岛素分别使Δ AVG增加了4倍和14倍（P < 0.0001）。F腿在低剂量胰岛素输注期间（组I）没有变化，但在组II中增加了112 ± 35%（P < 0.001）。在乙酰甲胆碱输注期间，组I和组II的Δ AVG分别缩小35.9 +/- 6.8%（P < 0.01）和20.5 +/- 4.8%（P < 0.05）。F腿的流速比单独使用胰岛素时的流速高224 +/- 30%（P < 0.01）和79 +/- 13%（P < 0.05）。因此，在乙酰甲胆碱流量调节期间，I组和II组的LGU分别增加了116 +/- 36%（P < 0.01）和47 +/- 11%（P < 0.05）。总之，高胰岛素血症时骨骼肌灌注可以作为IMGU的独立决定因素。
Insulin-mediated glucose uptake (IMGU) occurs principally in skeletal muscle. To directly examine whether skeletal muscle perfusion (F) can directly and independently modulate IMGU, we combined the hyperinsulinemic euglycemic clamp and leg balance techniques [leg glucose uptake (LGU) = arteriovenous glucose difference (Delta AVG) x F]. Young (< 40 yr) healthy lean subjects were studied during euglycemic hyperinsulinemia of similar to 23 mu U/ml (group I, n = 8) and similar to 950 mu U/ml (group II, n = 6). Femoral Delta AVG and leg F were measured, and LGU was calculated at baseline after similar to 200 min of steady-state hyperinsulinemia alone and during a superimposed intrafemoral artery infusion of methacholine hydrochloride designed to increase leg F two- to threefold. In groups I and II, insulin raised the Delta AVG similar to 4- and 14-fold, respectively (P < 0.0001). Leg F was unchanged during the low-dose insulin infusion (group I) but increased 112 +/- 35% in group II (P < 0.001). During methacholine infusion the Delta AVG narrowed 35.9 +/- 6.8% (P < 0.01) and 20.5 +/- 4.8% (P < 0.05) in groups I and II, respectively. Leg F rose 224 +/- 30% (P < 0.01) and 79 +/- 13% (P < 0.05) above the flow rate achieved with insulin alone. Thus, during methacholine flow modulation, LGU increased 116 +/- 36% (P < 0.01) and 47 +/- 11% (P < 0.05) in groups I and II, respectively. In conclusion, skeletal muscle perfusion during hyperinsulinemia can act as an independent determinant of IMGU.