Molecular gene organisation and secondary structure of the mitochondrial large subunit ribosomal RNA from the cultivated Basidiomycota Agrocybe aegerita:: a 13 kb gene possessing six unusual nucleotide extensions and eight introns
Molecular gene organisation and secondary structure of the mitochondrial large subunit ribosomal RNA from the cultivated Basidiomycota Agrocybe aegerita:: a 13 kb gene possessing six unusual nucleotide extensions and eight introns
复制标题
DOI：
10.1093/nar/27.7.1754
复制
发表时间：
1999-04-01
影响因子：
14.9
通讯作者：
Labarère, J
中科院分区：
文献类型：
--
作者：
Gonzalez, P;Barroso, G;Labarère, J
采用染色体行走法，获得了培养的绿绒担子菌线粒体LSU rRNA的完整基因序列和二级结构。A.aegerita LSU rRNA基因（13 526 nt）是迄今为止被描述的最长的基因，这是由于内含子数量最多（8个）和6个长核苷酸延伸。7个内含子属于I族，而内含子序列i5构成了真菌线粒体LSU rDNA中第一个典型的II族内含子。与迄今为止报道的大多数真菌LSU rDNA内含子一样，4个内含子（i5-i8）分布在与肽基转移酶活性相关的结构域V中。一个内含子（il）位于结构域I，三个内含子（i2-i4）位于结构域II。内含子i2-i8与位于LSU rnas相同位置的其他真菌、藻类或原生动物内含子具有同源性。其中一个（16）与大多数子囊菌或藻类LSU内含子位于相同的插入位点，提示可能来自共同祖先的遗传。相反，内含子ii位于迄今未报道的插入位点。在6个异常核苷酸延伸中，5个位于结构域I， 1个位于结构域v。这是首次报道的线粒体LSU rRNA基因序列和整个担子菌分裂的二级结构。
The complete gene sequence and secondary structure of the mitochondrial LSU rRNA from the cultivated Basidiomycota Agrocybe aegerita was derived by chromosome walking. The A.aegerita LSU rRNA gene (13 526 nt) represents, to date, the longest described, due to the highest number of introns (eight) and the occurrence of six long nucleotidic extensions. Seven introns belong to group I, while the intronic sequence i5 constitutes the first typical group II intron reported in a fungal mitochondrial LSU rDNA. As with most fungal LSU rDNA introns reported to date, four introns (i5-i8) are distributed in domain V associated with the peptidyl-transferase activity. One intron (il) is located in domain I, and three (i2-i4) in domain II. The introns i2-i8 possess homologies with other fungal, algal or protozoan introns located at the same position in LSU rDNAs. One of them (i6) is located at the same insertion site as most Ascomycota or algae LSU introns, suggesting a possible inheritance from a common ancestor. On the contrary, intron ii is located at a so-far unreported insertion site. Among the six unusual nucleotide extensions, five are located in domain I and one in domain V. This is the first report of a mitochondrial LSU rRNA gene sequence and secondary structure for the whole Basidiomycota division.