A rapid and sensitive method for the control of selected regulated and emerging mycotoxins in beer
A rapid and sensitive method for the control of selected regulated and emerging mycotoxins in beer
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DOI：
10.3920/wmj2017.2298
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Bartkevics, V
中科院分区：
文献类型：
--
作者：
Rozentale, I;Bogdanova, E.;Bartkevics, V
采用自动在线固相萃取（SPE）结合液相色谱/高分辨质谱（LC-HRMS）检测技术，建立了一种快速测定啤酒中12种霉菌毒素的方法，并根据欧盟（EU）指南中规定的性能标准进行了充分验证。真菌毒素检测实现了全扫描高分辨质谱与电喷雾电离在正离子模式在13分钟内。没有显着的基质干扰观察。新出现的真菌毒素（白僵菌素和恩镰孢菌素）和赭曲霉毒素A的最低检测限（LOD）在0.01至0.02微克/升之间，而总体LOD在0.01至0.30微克/升之间。该方法对啤酒样品的分析具有良好的线性（R-2>0.999）、日内和日间精密度，
A fast method using automated online solid-phase extraction (SPE) coupled to liquid-chromatography/high resolution mass spectrometric (LC-HRMS) detection for the determination of 12 mycotoxins in beer was developed and fully validated according to the performance criteria set in the European Union (EU) guidelines. Mycotoxin detection was achieved by full-scan HRMS with electrospray ionisation in positive ion mode within 13 min. No significant matrix interferences were observed. The lowest limits of detection (LODs) were achieved for emerging mycotoxins (beauvericin and enniatins) and ochratoxin A at levels between 0.01 and 0.02 mu g/l, while the overall LODs were in the range of 0.01-0.30 mu g/l. The elaborated method showed good performance in the analysis of beer samples with good linearity (R-2>0.999), intra-day and inter-day precision at the levels of